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CD27 antibody | B30C7

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Anti Pig CD27 Antibody, clone B30C7 thumbnail image 1 Anti Pig CD27 Antibody, clone B30C7 thumbnail image 2 Anti Pig CD27 Antibody, clone B30C7 thumbnail image 3 Anti Pig CD27 Antibody, clone B30C7 thumbnail image 4 Anti Pig CD27 Antibody, clone B30C7 thumbnail image 5 Anti Pig CD27 Antibody, clone B30C7 thumbnail image 6 Anti Pig CD27 Antibody, clone B30C7 thumbnail image 7
Anti Pig CD27 Antibody, clone B30C7 gallery image 1
Figure A. RPE conjugated mouse anti porcine CD3 (MCA5951PE) and APC conjugated mouse IgG1 isotype control (MCA928APC). Figure B. RPE conjugated mouse anti porcine CD3 (MCA5951PE) and APC conjugated mouse anti porcine CD27 (MCA5973APC). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD27 Antibody, clone B30C7 gallery image 2
Figure A. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti porcine CD8b (MCA5954PE) and FITC conjugated mouse anti porcine CD27 (MCA5973F). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD27 Antibody, clone B30C7 gallery image 3
Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:FITC (MCA5973F) and Mouse anti Pig wCD8a:RPE (MCA1223PE).
Anti Pig CD27 Antibody, clone B30C7 gallery image 4
Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27 (MCA5973GA) detected with Goat anti Mouse IgG:FITC (STAR117F), and Mouse anti Pig wCD8a:RPE (MCA1223PE).
Anti Pig CD27 Antibody, clone B30C7 gallery image 5
Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:RPE (MCA5973PE) and Mouse anti Pig wCD8a:FITC (MCA1223F).
Anti Pig CD27 Antibody, clone B30C7 gallery image 6
Figure A. FITC conjugated mouse anti porcine CD18a (MCA1972F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD18a (MCA1972F) and RPE conjugated mouse anti porcine CD27 (MCA5973PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD27 Antibody, clone B30C7 gallery image 7
Figure A. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse anti porcine CD18a (MCA1972F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse anti Pig CD27:FITC

Product Type
Monoclonal Antibody
Clone
B30C7
Isotype
IgG1
Specificity
CD27

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Product Code Applications Pack Size List Price Your Price Qty
MCA5973F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable (Reutner et al. 2012).

Porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells (Reutner et al. 2012).

Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells (Mair et al. 2013).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Porcine peripheral blood monocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD27 antibody

  1. Reutner, K. et al. (2012) Porcine CD27: identification, expression and functional aspects in lymphocyte subsets in swine.
    Dev Comp Immunol. 38: 321-31.
  2. Reutner, K. et al. (2013) CD27 expression discriminates porcine T helper cells with functionally distinct properties.
    Vet Res. 44: 18.
  3. Franzoni, G. et al. (2013) Assessment of the Phenotype and Functionality of Porcine CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (CSFV) and Virulent CSFV Challenge.
    Clin Vaccine Immunol. 20: 1604-16.
  4. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  5. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  6. Maciag, S.S. et al. (2022) The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
    Res Sq. Mar 24 [Epub ahead of print].

Synonyms
SWC2
UniProt
F1SL30

MCA5973F

150152 162164

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