CD5 antibody | 1H6/8

Mouse anti Pig CD5:FITC

Product Type
Monoclonal Antibody
Clone
1H6/8
Isotype
IgG2a
Specificity
CD5

Product Code Applications Pack Size List Price Your Price Qty
MCA2307F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB 0.1 mg loader
List Price Your Price
loader
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Mouse anti Pig CD5 antibody, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmuller et al. 1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody (Pescowitz et al. 1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine γ/δ T cells (Saalmuller et al. 1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+).

No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
ConA/PMA activated porcine peripheral blood cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG2a Negative Control:FITC

References for CD5 antibody

  1. Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD5: results from the Second International Swine CD Workshop.
    Vet Immunol Immunopathol. 60 (3-4): 269-73.
  2. Saalmüller, A. et al. (1998) Overview of the Second International Workshop to define swine cluster of differentiation (CD) antigens.
    Vet Immunol Immunopathol. 60 (3-4): 207-28.
  3. Doménech, N. et al. (2003) A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.
    Hybrid Hybridomics. 22 (3): 179-82.
  4. Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
    Arch Virol. 148 (12): 2307-23.
  5. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
  6. Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
    PLoS One. 14 (10): e0223647.
  7. Jarosz, Ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
    Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

RRID
AB_566839
UniProt
Q9GMA0
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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