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IgG antibody

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Anti Mouse IgG (Fc) Antibody thumbnail image 1 Anti Mouse IgG (Fc) Antibody thumbnail image 2 Anti Mouse IgG (Fc) Antibody thumbnail image 3 Anti Mouse IgG (Fc) Antibody thumbnail image 4 Anti Mouse IgG (Fc) Antibody thumbnail image 5 Anti Mouse IgG (Fc) Antibody thumbnail image 6 Anti Mouse IgG (Fc) Antibody thumbnail image 7
Anti Mouse IgG (Fc) Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) .used for the detection of murine IgG by ELISA.
Image caption:
PRO 2000 co-application induces increased mucosal antibody responses in mice. BALB/c mice (n = 7) were immunized four times vaginally with 12.5 μg gp140 +/− 1% PRO 2000; Time courses of the vaginal lavage IgA (A) and IgG (B) endpoint titres. gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p<0.05; ** p<0.01.

From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al.. (2011)
A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.
PLoS ONE 6(1): e15861.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Mouse IgG (Fc) Antibody gallery image 2
Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) used for the detection of murine IgG by ELISA.
Image caption:
Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n = 7) were primed intranasally with 10 μg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/− PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum IgG endpoint titres (A), or vaginal lavage IgG (B) and IgA (C) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO, combination of gp140 and PRO 2000.* p<0.05.

From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al.. (2011)
A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.
PLoS ONE 6(1): e15861.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Mouse IgG (Fc) Antibody gallery image 3
A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 Western Workflow™ and transferred to a PVDF membrane. Precision Plus Protein™ Prestained Standards were run in lane 1.

1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc Touch Imaging System.
Anti Mouse IgG (Fc) Antibody gallery image 4
Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) used to demonstrate bound Mouse anti tubulin antibody by western blotting.
Image caption:
Interaction of mga2 mutation with Cdk1 Tyr15 phosphorylation and Cdc13 nuclear accumulation. (A) Western blot for Tyr15-phosphorylated Cdc2, Cdc2, Cdc13 and α-tubulin in the WT, wee1Δ and mga2Δ strains. Each sample was loaded twice into consecutive lanes.

From: Wood E, Kume K, Navarro FJ, Nurse P.
Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
J Cell Sci. 2021 Jan 8:jcs.251769.
doi: 10.1242/jcs.251769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Mouse IgG (Fc) Antibody gallery image 5
Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) used as a secondary reagent to detect bound mouse antibodies in western blotting on human lysates.
Image caption:
(c) Left: Western blot of phosphorylated PI3K and Akt in control and PAM-treated 3D A549 spheroids. Total protein levels of PI3K and Akt were analyzed. β-Actin was used as a loading control. Right: quantification of phosphorylated PI3K and Akt normalized to the total amount of the same proteins. (d) Left: Western blot of phosphorylated ERK, p38, and JNK in control and PAM-treated 3D A549 cells. Total protein levels of ERK, p38, and JNK were analyzed. β-Actin was used as a loading control. Right: quantification of phosphorylated ERK, p38, and JNK normalized to the total amount of the same proteins.

From: Li Y, Lv Y, Zhu Y, Yang X, Lin B, Li M, Zhou Y, Tan Z, Choi EH, Wang J, Wang S, Liu Y.
Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways.
Oxid Med Cell Longev. 2022 Mar 18;2022:9014501.
doi: 10.1155/2022/9014501.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Mouse IgG (Fc) Antibody gallery image 6
Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG light chain antibody (STAR120P) used as a coating reagent in ELISA for determination of mouse antibody levels in serum samples.
Image caption:
Inflammation after immunization with unmodified mRNA is associated with dampened adaptive immune responses.
Blood was collected for analysis of HA specific antibody 21 days (B) and 42 days (C) after study start; 42 days after the study, spleens were collected and assessed for HA specific T cells by ELISPOT (D).

From: Wang Z, Jacobus EJ, Stirling DC, Krumm S, Flight KE, Cunliffe RF, Mottl J, Singh C, Mosscrop LG, Santiago LA, Vogel AB, Kariko K, Sahin U, Erbar S, Tregoning JS.
Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 2023 Oct 5;34:102045.
doi: 10.1016/j.omtn.2023.102045.
This image is from an open access article distributed under terms of a Creative Commons Attribution License
Anti Mouse IgG (Fc) Antibody gallery image 7
Published customer image:
Horseradish peroxidase conjugated Goat anti mouse IgG (Fc) polyclonal antibody (STAR120P) used to detect bound Mouse anti Cig2 antibodies in western blotting.
Image caption:
Overexpression of Cig2 cannot drive cell division in the absence of Cdc13.
b Western blots for Cig2 (upper) following thiamine withdrawal, with Ponceau-S staining (lower) of the same membrane region as the upper panel given as a loading control.

From: Basu S, Greenwood J, Jones AW, Nurse P.
Core control principles of the eukaryotic cell cycle.
Nature. 2022 Jul;607(7918):381-386.
doi: 10.1038/s41586-022-04798-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Mouse IgG (Fc):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

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Product Code Applications Pack Size List Price Your Price Qty
STAR120P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 1 mg loader
List Price Your Price
loader

Goat anti mouse IgG (Fc) polyclonal antibody reacts with mouse IgG at an epitope localised to the Fc region as assessed by immunoelectrophoresis and ELISA. Cross reactivity with IgA and IgM is negligible.

Goat anti mouse IgG (Fc) polyclonal antibody may cross react with IgG from other species.

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Western Blotting 1/5,000 1/50,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG antibody

  1. Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
    Parasitology. 136: 691-7.
  2. Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
    Biotechnol Prog. 26 (6): 1749-58.
  3. Wegmann, F. et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment
    PLoS One. 6(1):e15861.
  4. Wegmann F et al. (2015) The carbomer-lecithin adjuvant Adjuplex™ has potent immune activating properties and elicits protective adaptive immunity against influenza challenge in mice.
    Clin Vaccine Immunol. pii: CVI.00736-14.
  5. Liu, Z. et al. (2016) Partial protective immunity against toxoplasmosis in mice elicited by recombinant Toxoplasma gondii malate dehydrogenase.
    Vaccine. 34 (7): 989-94.
  6. Swaffer, M.P. et al. (2016) CDK Substrate Phosphorylation and Ordering the Cell Cycle.
    Cell. 167 (7): 1750-1761.e16.
  7. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. 50(6):e12391.
  8. Wood, E. et al. (2021) Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
    J Cell Sci. jcs.251769.
    9. View The Latest Product References
  9. Li, Y. et al. (2022) Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways.
    Oxid Med Cell Longev. 2022: 9014501.
  10. Wang, Z. et al. (2023) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
    Mol Ther Nucleic Acids. 34: 102045.
  11. Basu, S. et al. (2020) The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis.
    Curr Biol. 30 (5): 883-892.e4.
  12. Scotchman, E. et al. (2021) Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
    J Cell Sci. 134 (3)Feb 11 [Epub ahead of print].
  13. Basu, S. et al. (2022) Core control principles of the eukaryotic cell cycle.
    Nature. 607 (7918): 381-6.

ELISA

RRID
AB_567024
UniProt
P01869
P01865
P03987
P01867
P01864
P01868
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

STAR120P

151355 156805 167763

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