IgG antibody

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Goat anti Mouse IgG:FITC (Rat Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR70 F 0.5 mg
Goat anti Mouse IgG antibody recognizes mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues.

Goat IgG shows minimal binding to Fc receptors on rat tissue. We recommend diluting this product in buffer containing 10% normal rat serum to remove any residual cross-reactivity.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Mouse IgG.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Product Specific References

References for IgG antibody

  1. Voulgaraki, D. et al. (2005) Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor.
    Immunology. 115 (3): 337-46.
  2. Win, T.S. et al. (2009) Donor CD4 T cells contribute to cardiac allograft vasculopathy by providing help for autoantibody production.
    Circ Heart Fail. 2 (4): 361-9.
  3. Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.
    FASEB J. 26 (1): 51-62.
  4. Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.
    J Immunol. 188 (6): 2643-52.
  5. Fitzgerald, A.M. et al. (2012) The effects of transforming growth factor-β2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.
    Invest Ophthalmol Vis Sci. 53 (11): 7358-69.
  6. Gibbings, D.J. et al. (2007) CD8α is expressed by human monocytes and enhances FcγR-dependent responses.
    BMC Immunol. 8: 12.
  7. Chen, F. et al. (2015) Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting.
    J Genet Genomics. 42 (8): 437-44.
  8. Strazielle, N. et al. (2016) T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium.
    PLoS One. 11 (3): e0150945.
  9. Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
    Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print]
  10. Harper, I.G. et al. (2016) Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.
    Cell Rep. 15 (6): 1214-27.