IgG antibody

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/50	1/200

Flow Cytometry

Use 50ul of the suggested working dilution to label 10⁶ cells in 100ul.

References for IgG antibody

1. Voulgaraki, D. et al. (2005) Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor.
   Immunology. 115 (3): 337-46.
2. Win, T.S. et al. (2009) Donor CD4 T cells contribute to cardiac allograft vasculopathy by providing help for autoantibody production.
   Circ Heart Fail. 2 (4): 361-9.
3. Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.
   FASEB J. 26 (1): 51-62.
4. Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.
   J Immunol. 188 (6): 2643-52.
5. Fitzgerald, A.M. et al. (2012) The effects of transforming growth factor-β2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.
   Invest Ophthalmol Vis Sci. 53 (11): 7358-69.
6. Gibbings, D.J. et al. (2007) CD8α is expressed by human monocytes and enhances FcγR-dependent responses.
   BMC Immunol. 8: 12.
7. Chen, F. et al. (2015) Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting.
   J Genet Genomics. 42 (8): 437-44.
8. Strazielle, N. et al. (2016) T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium.
   PLoS One. 11 (3): e0150945.
View The Latest Product References
9. Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
   Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print]
10. Harper, I.G. et al. (2016) Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.
    Cell Rep. 15 (6): 1214-27.
11. Tyrsina, E. et al. (2019) Detection and quantification of VEGFR-1 in the nuclei of tumor cells by a new flow cytometry-based method.
    J Immunotoxicol. 16 (1): 74-81.
12. Niedżwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
    Pol J Vet Sci. 23 (1): 37-42.

RRID

AB_321914

UniProt

P01869

P01865

P01864

P01868

P03987

P01867

P01863

Entrez Gene

Igh-1a

Igh-1a

Igh-1a

Ighg2b

Ighg1

Ighg1

AI324046

GO Terms

GO:0003823 antigen binding

GO:0002455 humoral immune response mediated by circulating immunoglobulin

GO:0019731 antibacterial humoral response

GO:0042571 immunoglobulin complex, circulating

GO:0005886 plasma membrane

GO:0016021 integral to membrane

GO:0005576 extracellular region

GO:0001788 antibody-dependent cellular cytotoxicity

GO:0001798 positive regulation of type IIa hypersensitivity

View More GO Terms

GO:0001812 positive regulation of type I hypersensitivity

GO:0006910 phagocytosis, recognition

GO:0006911 phagocytosis, engulfment

GO:0006958 complement activation, classical pathway

GO:0050766 positive regulation of phagocytosis