- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
humoral immune response mediated by circulating immunoglobulin
antibacterial humoral response
immunoglobulin complex, circulating
integral to membrane
antibody-dependent cellular cytotoxicity
positive regulation of type IIa hypersensitivity
positive regulation of type I hypersensitivity
complement activation, classical pathway
positive regulation of phagocytosis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG antibody
Voulgaraki, D. et al. (2005) Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor.
Immunology. 115 (3): 337-46.
Win, T.S. et al. (2009) Donor CD4 T cells contribute to cardiac allograft vasculopathy by providing help for autoantibody production.
Circ Heart Fail. 2 (4): 361-9.
Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.
FASEB J. 26 (1): 51-62.
Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.
J Immunol. 188 (6): 2643-52.
Fitzgerald, A.M. et al. (2012) The effects of transforming growth factor-β2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.
Invest Ophthalmol Vis Sci. 53 (11): 7358-69.
Gibbings, D.J. et al. (2007) CD8α is expressed by human monocytes and enhances FcγR-dependent responses.
BMC Immunol. 8: 12.
Chen, F. et al. (2015) Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting.
J Genet Genomics. 42 (8): 437-44.
Strazielle, N. et al. (2016) T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium.
PLoS One. 11 (3): e0150945.
Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print]
Harper, I.G. et al. (2016) Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.
Cell Rep. 15 (6): 1214-27.
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