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CD8 Alpha antibody | MIL12

Mouse anti Pig wCD8 Alpha:RPE

Product Type
Monoclonal Antibody
Clone
MIL12
Isotype
IgG2a
Specificity
CD8 Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA1223PE
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SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Pig wCD8 alpha antibody, clone MIL12 recognizes an epitope on the alpha chain of porcine wCD8. Clone MIl12 was clustered at the Third International Swine CD Workshop (Haverson et al. 2001). Mouse anti Pig wCD8 alpha antibody, clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes (Saalmuller et al.2001).

Target Species
Pig
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Porcine mesenteric lymphocytes.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests
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List Price Your Price
Description Mouse IgG2a Negative Control:RPE

References for CD8 Alpha antibody

  1. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
    Kidney Int. 58: 838-50.
  2. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
    J Nutr. 131: 1520-7.
  3. Carter, D.B. et al. (2002) Phenotyping of transgenic cloned piglets.
    Cloning Stem Cells. 4: 131-45.
  4. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
    Kidney Int. 62: 654-67.
  5. Swamy, H.V. et al. (2003) Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on growth and immunological measurements of starter pigs, and the efficacy of a polymeric glucomannan mycotoxin adsorbent.
    J Anim Sci. 81: 2792-803.
  6. Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.
    Vet Pathol. 40: 395-404.
  7. Ostrowska, E. et al. (2004) Effects of dietary conjugated linoleic acid on haematological and humoral responses in the grower pig
    Austral J Agric Res. 55 (7): 711.
  8. Piva, A. et al. (2005) Activated carbon does not prevent the toxicity of culture material containing fumonisin B1 when fed to weanling piglets.
    J Anim Sci. 83 (8): 1939-47.
  9. View The Latest Product References
  10. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
    Vet Immunol Immunopathol. 104: 131-44.
  11. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
    Vet Microbiol. 129: 367-77.
  12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
    Animal. 2: 1575-84.
  13. Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
    Res Vet Sci. 91 (3): e68-72.
  14. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
    J Swine Health Prod. 20: 276–282.
  15. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
    BMC Genomics. 13: 488.
  16. Leifer, I. et al. (2012) Characterization of C-strain "Riems" TAV-epitope escape variants obtained through selective antibody pressure in cell culture.
    Vet Res. 43: 33.
  17. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
    BMC Vet Res. 8: 232.
  18. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
    J Anim Sci. 90: 649-56.
  19. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
    Cell Biol Int. 36 (12): 1233-8.
  20. Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
    Acta Vet Scand. 56: 42.
  21. Alex, P.J. (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model
    J Vaccines & Vaccination. 05 (05)
  22. Zeigler, B.M. et al. (2015) The development and validation of methods for evaluating the immune system in preweaning piglets.
    Food Chem Toxicol. 84: 197-207.
  23. Liermann, W. et al. (2017) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
    J Anim Physiol Anim Nutr (Berl). 101 (5): e414-e426.
  24. Hemmink, J.D. et al. (2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
    Vet Res. 47 (1): 103.
  25. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  26. Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
    Gene. 700: 105-9.
  27. Fogle, J.E. et al. (2019) Antibiotic Therapy Does Not Alter the Humoral Response to Vaccination for Porcine Circovirus 2 in Weaned Pigs.
    Vet Sci. 6 (2): 51.
  28. Nielsen, O.L. et al. (2022) A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study.
    APMIS. 130 (7): 359-70.
  29. Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
    Sci Rep. 12 (1): 15630.
  30. Wu, M.C. et al. (2023) A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs.
    Vet Res. 54 (1): 17.
  31. Bujňák, L. et al. (2023) The Effect of Dietary Humic Substances on Cellular Immunity and Blood Characteristics in Piglets
    Agriculture. 13 (3): 636.
  32. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
CD8
RRID
AB_323454

MCA1223PE

154472 1602

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