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IgG antibody

Goat anti Mouse IgG (Fc):FITC

Product Type
Polyclonal Antibody
Polyclonal IgG

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Goat anti mouse IgG (Fc) polyclonal antibody reacts with mouse IgG at an epitope localised to the Fc region as assessed by immunoelectrophoresis and ELISA. Cross reactivity with IgA and IgM is negligible.

Goat anti mouse IgG (Fc) polyclonal antibody may cross react with IgG from other species.

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/500
Immunohistology - Frozen 1/50 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG antibody

  1. Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
    Parasitology. 136: 691-7.
  2. Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
    Biotechnol Prog. 26 (6): 1749-58.
  3. Wegmann, F. et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment
    PLoS One. 6(1):e15861.
  4. Wegmann F et al. (2015) The carbomer-lecithin adjuvant Adjuplex™ has potent immune activating properties and elicits protective adaptive immunity against influenza challenge in mice.
    Clin Vaccine Immunol. pii: CVI.00736-14.
  5. Liu, Z. et al. (2016) Partial protective immunity against toxoplasmosis in mice elicited by recombinant Toxoplasma gondii malate dehydrogenase.
    Vaccine. 34 (7): 989-94.
  6. Swaffer, M.P. et al. (2016) CDK Substrate Phosphorylation and Ordering the Cell Cycle.
    Cell. 167 (7): 1750-1761.e16.
  7. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. 50(6):e12391.
  8. Wood, E. et al. (2021) Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
    J Cell Sci. jcs.251769.
  9. View The Latest Product References
  10. Li, Y. et al. (2022) Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways.
    Oxid Med Cell Longev. 2022: 9014501.
  11. Wang, Z. et al. (2023) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
    Mol Ther Nucleic Acids. 34: 102045.
  12. Basu, S. et al. (2020) The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis.
    Curr Biol. 30 (5): 883-892.e4.

Entrez Gene
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis


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