IgG antibody
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) .used for the detection of murine IgG by ELISA.
Image caption:
PRO 2000 co-application induces increased mucosal antibody responses in mice. BALB/c mice (n = 7) were immunized four times vaginally with 12.5 μg gp140 +/− 1% PRO 2000; Time courses of the vaginal lavage IgA (A) and IgG (B) endpoint titres. gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p<0.05; ** p<0.01.
From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al.. (2011)
A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.
PLoS ONE 6(1): e15861.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) used for the detection of murine IgG by ELISA.
Image caption:
Second-site immunization in the presence of PRO 2000 increases systemic and mucosal antibody responses. BALB/c mice (n = 7) were primed intranasally with 10 μg gp140 per animal in polycation and were boosted three times vaginally with gp140 +/− PRO 2000. Samples were collected 14 days after the final vaginal immunization. Serum IgG endpoint titres (A), or vaginal lavage IgG (B) and IgA (C) endpoint titres normalized to the total IgG or IgA content or each sample, respectively, are displayed. gp140 + PRO, combination of gp140 and PRO 2000.* p<0.05.
From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al.. (2011)
A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.
PLoS ONE 6(1): e15861.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System
Horseradish peroxidase conjugated Goat anti Mouse IgG antibody (STAR120P) used to demonstrate bound Mouse anti tubulin antibody by western blotting.
Image caption:
Interaction of mga2 mutation with Cdk1 Tyr15 phosphorylation and Cdc13 nuclear accumulation. (A) Western blot for Tyr15-phosphorylated Cdc2, Cdc2, Cdc13 and α-tubulin in the WT, wee1Δ and mga2Δ strains. Each sample was loaded twice into consecutive lanes.
From: Wood E, Kume K, Navarro FJ, Nurse P.
Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
J Cell Sci. 2021 Jan 8:jcs.251769.
doi: 10.1242/jcs.251769.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti mouse IgG (Fc) polyclonal antibody reacts with mouse IgG at an epitope localised to the Fc region as assessed by immunoelectrophoresis and ELISA. Cross reactivity with IgA and IgM is negligible.
Goat anti mouse IgG (Fc) polyclonal antibody may cross react with IgG from other species.
Goat anti mouse IgG (Fc) polyclonal antibody may cross react with IgG from other species.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01869
- P01865
- P03987
- P01867
- P01864
- P01868
- P01863
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/500 | |
| Immunohistology - Frozen | 1/50 | 1/500 | |
| ELISA | 1/10,000 | 1/100,000 | |
| Western Blotting | 1/5,000 | 1/50,000 | |
| ELISA | 1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
Parasitology. 136: 691-7. -
Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
Biotechnol Prog. 26 (6): 1749-58. -
Wegmann, F. et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment
PLoS One. 6(1):e15861. -
Wegmann F et al. (2015) The carbomer-lecithin adjuvant Adjuplex™ has potent immune activating properties and elicits protective adaptive immunity against influenza challenge in mice.
Clin Vaccine Immunol. pii: CVI.00736-14. -
Liu, Z. et al. (2016) Partial protective immunity against toxoplasmosis in mice elicited by recombinant Toxoplasma gondii malate dehydrogenase.
Vaccine. 34 (7): 989-94. -
Swaffer, M.P. et al. (2016) CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Cell. 167 (7): 1750-1761.e16. -
Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. 50(6):e12391. -
Wood, E. et al. (2021) Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
J Cell Sci. jcs.251769.
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