IgA antibody | K84 2F9
Mouse anti Bovine IgA antibody, clone K84 2F9 used for the measurement of Tci-L4-ES-specific IgA responses in sheep by ELISA.
Image caption:
The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression. Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 μg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A) Faecal egg count (FEC) data over the course of the experimental infection. (B) Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C) Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a significant difference between Con A + PBS vs. PBS-stimulated cultures; b significant difference between ConA + ES vs. PBS-stimulated cultures; c significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P < 0.05, ** P < 0.01, ***P < 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).
From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine/Ovine IgA
- Product Type
- Monoclonal Antibody
- Clone
- K84 2F9
- Isotype
- IgG1
- Specificity
- IgA
Antibody Quality and Performance Guarantee
| Mouse anti Bovine IgA antibody, clone K84 2F9 recognizes bovine and ovine IgA. No cross reactivity is observed with Bovine or Ovine IgG and IgM, |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine IgA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | 1/80,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Bovine IgA | MCA2438GA | E IP | 0.1 mg |
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| Description | Mouse anti Bovine IgA | ||||||
| Mouse anti Bovine IgA:HRP | MCA2438P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgA:HRP | ||||||
| Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg |
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| Description | Mouse anti Bovine IgG | ||||||
| Mouse anti Bovine IgG:HRP | MCA2439P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgG:HRP | ||||||
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
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| Description | Mouse anti Bovine IgG1 | ||||||
| Mouse anti Bovine IgG1:HRP | MCA2440P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgG1:HRP | ||||||
| Mouse anti Bovine IgG2 | MCA2441GA | E F IP | 0.1 mg |
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| Description | Mouse anti Bovine IgG2 | ||||||
| Mouse anti Bovine IgG2:HRP | MCA2441P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgG2:HRP | ||||||
| Mouse anti Bovine IgM | MCA2443GA | E F IP R | 0.1 mg |
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| Description | Mouse anti Bovine IgM | ||||||
| Mouse anti Sheep IgE | MCA5941GA | C E IF WB | 0.1 mg |
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| Description | Mouse anti Sheep IgE | ||||||
| Mouse anti Bovine IgG1 | MCA627GA | E | 0.1 mg |
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| Description | Mouse anti Bovine IgG1 | ||||||
References for IgA antibody
-
Estes, D.M. et al. (1998) Effects of type I/type II interferons and transforming growth factor-beta on B-cell differentiation and proliferation. Definition of costimulation and cytokine requirements for immunoglobulin synthesis and expression.
Immunology. 95 (4): 604-11. -
Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
Microbes Infect. 10: 1370-8. -
Parreño, V.et al. (2004) Modulation by colostrum-acquired maternal antibodies of systemic and mucosal antibody responses to rotavirus in calves experimentally challenged with bovine rotavirus.
Vet Immunol Immunopathol. 100 (1-2): 7-24. -
Hassan, M. et al. (2011) The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection.
Vet Res. 42: 46. -
Stanley AC et al. (2004) Intranasal immunisation with Toxoplasma gondii tachyzoite antigen encapsulated into PLG microspheres induces humoral and cell-mediated immunity in sheep.
Vaccine. 22 (29-30): 3929-41. -
Tzelos, T. et al. (2016) A preliminary proteomic characterisation of extracellular vesicles released by the ovine parasitic nematode, Teladorsagia circumcincta..
Vet Parasitol. 221: 84-92. -
Mahajan, A. et al. (2005) Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue.
Cell Tissue Res. 321 (3): 365-74. -
Suraud, V. et al. (2007) Differential expression of homing receptors and vascular addressins in tonsils and draining lymph nodes: Effect of Brucella infection in sheep.
Vet Immunol Immunopathol. 115 (3-4): 239-50.
View The Latest Product References
-
McNeilly, T.N. et al. (2007) Simple methods for measurement of bovine mucosal antibody responses in vivo.
Vet Immunol Immunopathol. 118 (1-2): 160-7. -
McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling.
Vaccine. 28 (5): 1412-21. -
McNeilly, T.N. et al. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge.
Vaccine. 28 (5): 1422-8. -
Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium.
Cell Microbiol. 11 (1): 121-37. -
McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 44: 70. -
Nisbet, A.J. et al. (2016) Protection of ewes against Teladorsagia circumcincta. infection in the periparturient period by vaccination with recombinant antigens.
Vet Parasitol. 228: 130-6. -
Queiroga, M.C. (2018) Local and systemic humoral response to ovine mastitis caused by Staphylococcus epidermidis.
SAGE Open Med. 6: 2050312118801466. -
McBean, D. et al. (2023) Immune markers in goats selected for reduced gastrointestinal nematode egg count under artificial infection conditions.
Vet Parasitol. 322: 110004. -
Palarea-Albaladejo, J. et al. (2024) A curated multivariate approach to study efficacy and optimisation of a prototype vaccine against teladorsagiasis in sheep.
Vet Res Commun. 48 (1): 367-79.
MCA628GA
1607
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