IgA Antibody | K84 2F9

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IgA Antibody | K84 2F9 gallery image 1

Purified Bovine IgA detected with Mouse anti Bovine/Ovine IgA (MCA628GA) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B)

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IgA Antibody | K84 2F9 gallery image 2

Published customer image:
Mouse anti Bovine IgA antibody, clone K84 2F9 used for the measurement of Tci-L4-ES-specific IgA responses in sheep by ELISA.
Image caption:
The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression.?Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta?L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 µg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A)?Faecal egg count (FEC) data over the course of the experimental infection. (B)?Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C)?Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta?infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a?significant difference between Con A + PBS vs. PBS-stimulated cultures; b?significant difference between ConA + ES vs. PBS-stimulated cultures; c?significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P?< 0.05, ** P?< 0.01, ***P?< 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).

From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..

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  • Mouse anti Bovine/Ovine IgA
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  • Product Type
    Monoclonal Antibody
  • Clone
    K84 2F9
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA628GAEdatasheet pdfdatasheet pdf0.1 mg
    MCA628GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine IgA antibody, clone K84 2F9 recognises bovine and ovine IgA. No cross reactivity is observed with Bovine or Ovine IgG and IgM,
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Bovine IgA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/50001/80,000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Clone Applications Sizes available
    IgA Antibody : Purified K84 2F9 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine IgA:HRPMCA2438P0.25 mgE
          MCA2438P
          Mouse anti Bovine IgG:HRPMCA2439P0.25 mgE
          MCA2439P
          Mouse anti Bovine IgG1:HRPMCA2440P0.25 mgE
          MCA2440P
          Mouse anti Bovine IgG2:HRPMCA2441P0.25 mgE
          MCA2441P
          Mouse anti Bovine IgAMCA2438GA0.1 mgE, IP
          MCA2438GA
          Mouse anti Bovine IgG1MCA2440GA0.1 mgC, E, F, IP
          MCA2440GA
          Mouse anti Bovine IgG2MCA2441GA0.1 mgE, F, IP
          MCA2441GA
          Mouse anti Bovine IgMMCA2443GA0.1 mgE, F, IP, R
          MCA2443GA
          Mouse anti Sheep IgEMCA5941GA0.1 mgC, E, IF, WB
          MCA5941GA
          Mouse anti Bovine IgG1MCA627GA0.1 mgE
          MCA627GA

          Recommended Positive Controls

            Histology Controls

              References

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