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IgG antibody | IL-A2

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Anti Bovine IgG Antibody, clone IL-A2 thumbnail image 1 Anti Bovine IgG Antibody, clone IL-A2 thumbnail image 2 Anti Bovine IgG Antibody, clone IL-A2 thumbnail image 3
Anti Bovine IgG Antibody, clone IL-A2 gallery image 1
Bovine IgG (PBP002) detected using Mouse anti Bovine IgG (MCA2439GA) and Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B) as the detection reagents.
Anti Bovine IgG Antibody, clone IL-A2 gallery image 2
Bovine IgG (PBP002) detected using Mouse anti Bovine IgG:HRP (MCA2439P) as the detection reagent.
Anti Bovine IgG Antibody, clone IL-A2 gallery image 3
Published customer image:
Horseradish peroxidase conjugated Mouse anti Bovine IgG antibody, clone IL-A2 (MCA2439P) used to evaluate bovine IgG2 levels in serum samples by ELISA.
Image caption:
Antibody profile of S19 and RB51 prime vaccinated, and RB51 revaccinated cattle measured by I-ELISA using S19 and RB51 whole-cell antigens.
The results are expressed as mean. Data for total IgG, IgG1 and IgG2 are shown. Vaccinations were indicated by arrows. Lowercase letters indicate statistical difference between days on same group (one-way ANOVA followed by paired t-test).

From: Dorneles EMS, Lima GK, Teixeira-Carvalho A, Araújo MSS, Martins-Filho OA, Sriranganathan N, et al. (2015)
Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS ONE 10(9): e0136696.
doi: 10.1371/journal.pone.0136696
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Bovine IgG:HRP

Product Type
Monoclonal Antibody
Clone
IL-A2
Isotype
IgG1
Specificity
IgG

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Product Code Applications Pack Size List Price Your Price Qty
MCA2439P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.25 mg loader
List Price Your Price
loader

Mouse anti Bovine IgG, clone IL-A2 is specific for bovine Immunoglobulin G (IgG). The specificity of this antibody for bovine IgG1 and IgG2 has been demonstrated by ELISA.

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified bovine IgG
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/300 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG antibody

  1. Naessens, J. et al. (1988) Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.
    Immunology. 63 (4): 569-74.
  2. Buza, J. et al. (1997) CD5+ B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolese- infected cattle.
    Immunology 92: 226-33.
  3. VanDissel, J.T. et al. (2005) Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data.
    J Med Microbiol. 54 (Pt 2): 197-205.
  4. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.
  5. Tabynov, K. et al. (2016) Prime-booster vaccination of cattle with an influenza viral vector Brucella abortus vaccine induces a long-term protective immune response against Brucella abortus infection.
    Vaccine. 34 (4): 438-44.

RRID
AB_1605075

MCA2439P

153614 162177

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