IgE antibody | 1E7

Mouse anti Sheep IgE

Product Type
Monoclonal Antibody
Clone
1E7
Isotype
IgG2a
Specificity
IgE

Product Code Applications Pack Size List Price Your Price Qty
MCA5941GA
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SDS Safety Datasheet SDS
C E IF WB 0.1 mg loader
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loader
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Mouse anti Sheep IgE, clone 1E7, recognizes ovine Immunoglobulin E (IgE) and does not cross react with ovine IgM, IgA, IgG1 or IgG2.

IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman et al. 1997).

IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep by Haemonchus contortus (Vervelde et al. 1997 and Kooyman et al. 1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello et al. 2007 and Kooyman et al. 2002).

In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Horse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant ovine IgE1-2 expressed in E.coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/2000
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine IgA MCA2438GA E IP 0.1 mg loader
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Description Mouse anti Bovine IgA
Mouse anti Bovine IgA:HRP MCA2438P E 0.25 mg loader
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Description Mouse anti Bovine IgA:HRP
Mouse anti Bovine IgG MCA2439GA E IP 0.1 mg loader
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Description Mouse anti Bovine IgG
Mouse anti Bovine IgG:HRP MCA2439P E 0.25 mg loader
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Description Mouse anti Bovine IgG:HRP
Mouse anti Bovine IgG1 MCA2440GA C E F IP 0.1 mg loader
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Description Mouse anti Bovine IgG1
Mouse anti Bovine IgG1:HRP MCA2440P E 0.25 mg loader
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Description Mouse anti Bovine IgG1:HRP
Mouse anti Bovine IgG2 MCA2441GA E F IP 0.1 mg loader
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Description Mouse anti Bovine IgG2
Mouse anti Bovine IgG2:HRP MCA2441P E 0.25 mg loader
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Description Mouse anti Bovine IgG2:HRP
Mouse anti Bovine IgM MCA2443GA E F IP R 0.1 mg loader
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Description Mouse anti Bovine IgM
Mouse anti Bovine IgG1 MCA627GA E 0.1 mg loader
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Description Mouse anti Bovine IgG1

References for IgE antibody

  1. Bricarello, P.A. et al. (2007) Field study on nematode resistance in Nelore-breed cattle.
    Vet Parasitol.148:272-8
  2. Vervelde, L. et al. (2003) Vaccination-induced protection of lambs against the parasitic nematode Haemonchus contortus correlates with high IgG antibody responses to the LDNF glycan antigen.
    Glycobiology 13:795-804
  3. Kooyman, F.N. et al. (2002) Serum immunoglobulin E response in calves infected with the lungworm Dictyocaulus viviparus and its correlation with protection.
    Parasite Immunol. 24:47-56
  4. Kooyman, F.N. et al. (1997) Production of a monoclonal antibody specifc for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection.
    Parasitology 114:395-406
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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