IgG2 antibody | IL-A73
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E ResetMouse anti Bovine IgG2:HRP
- Product Type
- Monoclonal Antibody
- Clone
- IL-A73
- Isotype
- IgG2a
- Specificity
- IgG2
Mouse anti Bovine IgG2, clone IL-A73, is a monoclonal antibody specific for bovine IgG2 and does not recognise other bovine immunoglobulin classes. While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races. Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 (Campbell et al. 1998). |
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified bovine IgG2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100 | 1/1000 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
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Description | AbGUARD® HRP Stabilizer Plus | ||||||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
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Description | AbGUARD® HRP Stabilizer Plus | ||||||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
List Price | Your Price | ||||||
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Description | AbGUARD® HRP Stabilizer Plus | ||||||
TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TMB Signal+ | ||||||
TMB Core | BUF056A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TMB Core | ||||||
TMB Core+ | BUF062A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TMB Core+ |
References for IgG2 antibody
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Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
J Leukoc Biol. 63 (5): 567-74. -
Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
Vet Immunol Immunopathol. 24 (3): 267-83. -
Williams, D.J. et al. (1996) The role of anti-variable surface glycoprotein antibody responses in bovine trypanotolerance.
Parasite Immunol. 18 (4): 209-18. -
Hecker, Y.P. et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
Vet Immunol Immunopathol. 162 (3-4): 142-53. -
Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS One. 10 (9): e0136696. -
Pereyra, R. et al. (2019) Evidence of reduced vertical transmission of Neospora caninum. associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition.
Vet Immunol Immunopathol. 208: 53-57. -
Jaramillo, J.O. et al. (2019) Immunisation of cattle against Babesia bovis. combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection.
Ticks Tick Borne Dis. 10 (6): 101270. -
Villa-Mancera, A. et al. (2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica. induces protective cellular and humoral immune responses in sheep.
Vet Parasitol. 289: 109340.
View The Latest Product References
-
Di Giacomo, S. et al. (2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in Cattle.
Viruses. 14 (8): 1781. -
Noble, A. et al. (2024) Development of bovine IgG3-specific assays using a novel recombinant single-domain binding reagent
Veterinary Immunology and Immunopathology. : 110852.
- RRID
- AB_1605096
MCA2441P
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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