IgG1 antibody | IL-A60

Mouse anti Bovine IgG1:HRP

Product Type
Monoclonal Antibody
Clone
IL-A60
Isotype
IgG1
Specificity
IgG1

Product Code Applications Pack Size List Price Your Price Qty
MCA2440P
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E 0.25 mg loader
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Mouse anti Bovine IgG1 antibody, clone IL-A60 recognizes bovine IgG1. Mouse anti Bovine IgG1 antibody, clone IL-A60 immunoprecipitates a protein band of 55-59 kDa, consistent with the heavy chain of bovine IgG1 (Campbell et al. 1998).

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified bovine Immunoglobulin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core

References for IgG1 antibody

  1. Di Giacomo, S. et al. (2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in Cattle.
    Viruses. 14 (8): 1781.
  2. Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
    J Leukoc Biol. 63 (5): 567-74.
  3. Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
    Vet Immunol Immunopathol. 24 (3): 267-83.
  4. Williams, D.J. et al. (1996) The role of anti-variable surface glycoprotein antibody responses in bovine trypanotolerance.
    Parasite Immunol. 18 (4): 209-18.
  5. Hecker YP et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
    Vet Immunol Immunopathol. 162 (3-4): 142-53.
  6. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.
  7. Hecker, Y.P. et al. (2019) Immune response to Neospora caninum live tachyzoites in prepubertal female calves.
    Parasitol Res. 118 (10): 2945-55.
  8. Pereyra, R. et al. (2019) Evidence of reduced vertical transmission of Neospora caninum. associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition.
    Vet Immunol Immunopathol. 208: 53-57.
  9. View The Latest Product References
  10. Jaramillo, J.O. et al. (2019) Immunisation of cattle against Babesia bovis. combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection.
    Ticks Tick Borne Dis. 10 (6): 101270.
  11. Villa-Mancera, A. et al. (2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica. induces protective cellular and humoral immune responses in sheep.
    Vet Parasitol. 289: 109340.

RRID
AB_1605095
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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