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BrdU Staining of Cells with Anti-BrdU Antibody (clone Bu20a), by Flow Cytometry
For use with flow cytometry tested Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483).
This method provides a general procedure for use with the majority of Bio-Rad reagents. In some cases specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with product and batch specific information provided with each vial. Note that a certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques - these are guidelines only and may need to be adjusted for particular applications.
The acid treatment to unwind the DNA may affect surface immunophenotyping. Staining of cells with BrdU using DNAse I may be applicable if this is required.
Appropriate controls should be carried out for flow cytometry, consider including the following:
For all multicolor flow cytometry experiments include compensation controls and fluorescence minus one (FMO) controls, which assist with identifying gating boundaries.