PCNA Antibody | PC10

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PCNA Antibody | PC10 gallery image 1

Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (MCA1588)

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PCNA Antibody | PC10 gallery image 2

Staining of KM-H2 cells with Mouse anti PCNA:FITC (MCA1558F) permeabilised with Leucoperm (BUF09) and methanol

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PCNA Antibody | PC10 gallery image 3

K562 erythroleukemic whole cell lysate was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Mouse anti Human PCNA (MCA1558) was used as primary antibody followed by a Goat anti Mouse IgG (H/L):HRP secondary antibody at a dilution of 1/10,000 (STAR207P) was used as the secondary antibody. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale

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PCNA Antibody | PC10 gallery image 4

K562 erythroleukemic whole cell lysate was run in lane 2 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. MCA1558P Mouse anti Human PCNA:HRP conjugate was used as a direct label at a 1/1000 dilution. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale

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PCNA Antibody | PC10 gallery image 5

Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green

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  • PCNA Antibody | PC10 thumbnail image 1
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  • PCNA Antibody | PC10 thumbnail image 5
  • Mouse anti PCNA:FITC
  • Mouse anti PCNA
  • Mouse anti PCNA:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    PC10
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1558FF *datasheet pdfdatasheet pdf0.1 mg
    MCA1558F
    MCA1558C, F *, IP, P, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA1558
    MCA1558PWBdatasheet pdfdatasheet pdf0.1 mg
    MCA1558P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

      PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006).
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      VertebratesExpected from Sequence
      InvertebratesExpected from Sequence
      Ferretyes
      Chickenyes
      Rabbityes
      Xenopusyes
      Rhesus Monkeyyes
      Hamsteryes
      Atlantic Salmonyes
      Humanyes
      Mouseyes
      Horseyes
      Sheepyes
      Dogyes
      Catyes
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Rat PCNA made in the protein A expression vector pR1T2T
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch
    • GO Terms
      translesion synthesis
      positive regulation of deoxyribonuclease activity
      heart development
      DNA polymerase processivity factor activity
      response to cadmium ion
      regulation of DNA replication
      DNA replication
      PCNA-p21 complex
      PCNA complex
      response to lipid
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin1/501/100
      Immunoprecipitation
      Western Blotting1/1000
      Flow Cytometry(1)1/100
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections.
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional PCNA Antibody Formats

    Formats Clone Applications Sizes available
    PCNA Antibody : HRP PC10 WB 0.1 mg
    PCNA Antibody : FITC PC10 F * 0.1 mg
    PCNA Antibody : Purified PC10 C, F *, IP, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA1210F0.1 mgF
        MCA1210F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA1210100 TestsF
        MCA1210

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Tonsil
              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Paraffin

              References

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