GAPDH Antibody

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GAPDH Antibody gallery image 1

Western blot analysis of whole cell lysate from HeLa, human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH (AHP1628) at 0.125(A), 0.25(B) and 0.5(C) ug/ml

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GAPDH Antibody gallery image 2

Immunohistochemical staining of human liver with Rabbit anti Human GAPDH (AHP1628)

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GAPDH Antibody gallery image 3

HeLa (lane 2) and HepG2 (lane 3) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. Rabbit anti Human GAPDH (AHP1628) was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP MCA6003P was used as the secondary antibody at a dilution of 1/5000 . Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure

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GAPDH Antibody gallery image 4

Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green

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  • GAPDH Antibody thumbnail image 1
  • GAPDH Antibody thumbnail image 2
  • GAPDH Antibody thumbnail image 3
  • GAPDH Antibody thumbnail image 4
  • Rabbit anti GAPDH (N-Terminal)
  • Rabbit anti GAPDH (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1628P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1628
    AHP1628TP *, WBdatasheet pdfdatasheet pdf50 µg
    AHP1628T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti GAPDH (N-Terminal) antibody recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair.

      GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human GADPH (N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to a 16 amino acid sequence from near the amino-terminus of GAPDH.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      perinuclear region of cytoplasm
      peptidyl-cysteine S-trans-nitrosylation
      nucleus
      membrane
      oxidation-reduction process
      glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
      peptidyl-cysteine S-nitrosylase activity
      protein stabilization
      gluconeogenesis
      protein binding
      glycolysis
      neuron apoptosis
      cytosol
      NAD binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)10ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)10ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human liver.
    • Histology Positive Control Tissue
      Human liver tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Rabbit anti GAPDH antibody detects a band of approximately 40kDa in HeLa cell lysates.
    • Western Blotting
      AHP1628 detects a band of approximately 40kDa in HeLa cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional GAPDH Antibody Formats

    Formats Applications Sizes available
    GAPDH Antibody : Purified P *, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human liver.
              Human liver tissue

              References

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