CD3 antibody | PPT3
Mouse anti Pig CD3:FITC
Mouse anti Pig CD3:Low Endotoxin
Mouse anti Pig CD3
Mouse anti Pig CD3:RPE
- Product Type
- Monoclonal Antibody
- Clone
- PPT3
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA5951F | F | 0.1 mg |
|
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| MCA5951EL | C F FN IP WB | 0.5 mg |
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| MCA5951GA | C F IP WB | 0.1 mg |
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| MCA5951PE | F | 100 Tests |
|
Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3ε, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3ε designated as CD3c (Pescovitz, M.D., et al. 1998).
CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells.
Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate α/β T-cells (Kirkham P.A., et al. 1996).
Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).
CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells.
Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate α/β T-cells (Kirkham P.A., et al. 1996).
Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat Horse Human Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Porcine PBMCs
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Lymph node cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q7YRN2
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- Regulatory
- For research purposes only
Applications of CD3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/200 | |
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig CD25 | MCA1736GA | C F | 0.1 mg |
|
|
| Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests |
|
|
| Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests |
|
|
| Mouse anti Pig wCD8 Alpha:FITC | MCA1223F | F | 0.1 mg |
|
|
| Mouse anti Pig CD4 Alpha:FITC | MCA1749F | F | 0.1 mg |
|
|
| Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests |
|
|
| Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests |
|
|
| Mouse anti Pig wCD8 Alpha:FITC | MCA1223F | F | 0.1 mg |
|
|
| Mouse anti Pig CD4 Alpha:FITC | MCA1749F | F | 0.1 mg |
|
|
| Mouse anti Pig CD335:Alexa Fluor® 488 | MCA5972A488 | F | 100 Tests/1ml |
|
|
| Mouse anti Pig CD27:FITC | MCA5973F | F | 0.1 mg |
|
|
| Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests |
|
|
| Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests |
|
|
| Mouse anti Pig CD335:APC | MCA5972APC | F | 100 Tests |
|
|
| Mouse anti Pig CD27:APC | MCA5973APC | F | 100 Tests |
|
|
| Mouse anti Pig CD27:RPE | MCA5973PE | F | 100 Tests |
|
|
| Mouse anti Pig wCD8 Alpha:FITC | MCA1223F | F | 0.1 mg |
|
|
| Mouse anti Pig CD25 | MCA1736GA | C F | 0.1 mg |
|
|
| Mouse anti Pig CD4 Alpha:FITC | MCA1749F | F | 0.1 mg |
|
Product Specific References
References for CD3 antibody
-
Yang, H. et al. (1996) Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes.
Immunology. 88 (4): 577-85. -
Kirkham, P.A. et al. (1996) Porcine CD3 epsilon: its characterization, expression and involvement in activation of porcine T lymphocytes.
Immunology. 87 (4): 616-23. -
Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60: 261-8. -
Forberg H et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
PLoS One. 9 (6): e100619.
Further Reading
-
Guy, C.S. & Vignali, D.A. (2009) Organization of proximal signal initiation at the TCR:CD3 complex.
Immunol Rev. 32: 7-21.
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