CD3 antibody | PPT3
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F ResetMouse anti Pig CD3
- Product Type
- Monoclonal Antibody
- Clone
- PPT3
- Isotype
- IgG1
- Specificity
- CD3
- Region
- EPSILON
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| Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3ε, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3ε designated as CD3c (Pescovitz, M.D., et al. 1998). CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells. Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate α/β T-cells (Kirkham P.A., et al. 1996). Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat Horse Human Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine PBMCs
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Lymph node cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD335:Alexa Fluor® 488 | MCA5972A488 | F | 100 Tests/1ml |
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| Description | Mouse anti Pig CD335:Alexa Fluor® 488 | ||||||
| Mouse anti Pig CD335:APC | MCA5972APC | F | 100 Tests |
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| Description | Mouse anti Pig CD335:APC | ||||||
| Mouse anti Pig CD27:APC | MCA5973APC | F | 100 Tests |
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| Description | Mouse anti Pig CD27:APC | ||||||
| Mouse anti Pig CD27:FITC | MCA5973F | F | 0.1 mg |
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| Description | Mouse anti Pig CD27:FITC | ||||||
| Mouse anti Pig CD27:RPE | MCA5973PE | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD27:RPE | ||||||
| Mouse anti Pig CD4 Alpha:FITC | MCA1749F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD4 Alpha:FITC | ||||||
| Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests |
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| Description | Mouse anti Pig CD4 Alpha:RPE | ||||||
| Mouse anti Pig wCD8 Alpha:FITC | MCA1223F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig wCD8 Alpha:FITC | ||||||
| Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests |
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| Description | Mouse anti Pig wCD8 Alpha:RPE | ||||||
References for CD3 antibody
-
Yang, H. et al. (1996) Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes.
Immunology. 88 (4): 577-85. -
Kirkham, P.A. et al. (1996) Porcine CD3 epsilon: its characterization, expression and involvement in activation of porcine T lymphocytes.
Immunology. 87 (4): 616-23. -
Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60: 261-8. -
Forberg H et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
PLoS One. 9 (6): e100619. -
Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
J Immunol. Oct 08 [Epub ahead of print]. -
Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
Front Immunol. 13: 950194.
Further Reading
-
Guy, C.S. & Vignali, D.A. (2009) Organization of proximal signal initiation at the TCR:CD3 complex.
Immunol Rev. 32: 7-21. -
Maciag, S.S. et al. (2022) The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
Ref Sq. Mar 24 [Epub ahead of print].
- UniProt
- Q7YRN2
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
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