CD335 antibody | VIV-KM1
Mouse anti Pig CD335:APC
- Product Type
- Monoclonal Antibody
- Clone
- VIV-KM1
- Isotype
- IgG1
- Specificity
- CD335
Mouse anti Pig CD335 antibody, clone VIV-KM1 recognizes the porcine homologue of human CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development. CD335 is expressed by human natural killer cells (Sivori et al.1997). The development of monoclonal antibodies to bovine CD335 (clone AKS1) (Storset et al. 2004) and ovine CD335 (clone EC1.1) (Connelley et al.2011) have enabled researchers to identify and better understand ruminant NK cells. Mouse anti Pig CD335 antibody, clone VIV-KM is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the pig immune system and aid in the understanding of the role of NK cells in host pathogen defense. Porcine CD335 is not expressed by all NK cells and expression may be influenced by cytokine production (Mair et al. 2012). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Fusion protein consisting of the extracellular region of porcine CD335.
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) APC 650 661 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:APC |
Source Reference
-
Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
Eur J Immunol. 42 (5): 1261-71.
References for CD335 antibody
-
Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 44: 13. -
Forberg, H. et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
PLoS One. 9 (6): e100619. -
Yang, G. et al. (2017) Characterizing porcine invariant natural killer T cells: A comparative study with NK cells and T cells.
Dev Comp Immunol. 76: 343-51. -
Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
Virol J. 20 (1): 181.
Further Reading
-
Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation.
J Exp Med. 186 (7): 1129-36. -
Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.
Eur J Immunol. 34 (3): 669-76. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37.
- Synonyms
- NKp46
MCA5972APC
151682 INN1612If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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