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CD27 antibody | B30C7

Mouse anti Pig CD27:APC

Product Type
Monoclonal Antibody
Clone
B30C7
Isotype
IgG1
Specificity
CD27

Product Code Applications Pack Size List Price Your Price Qty
MCA5973APC
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SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable (Reutner et al. 2012).

Porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells (Reutner et al. 2012).

Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells (Mair et al. 2013).

Target Species
Pig
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Porcine peripheral blood monocytes.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:APC

References for CD27 antibody

  1. Reutner, K. et al. (2012) Porcine CD27: identification, expression and functional aspects in lymphocyte subsets in swine.
    Dev Comp Immunol. 38: 321-31.
  2. Reutner, K. et al. (2013) CD27 expression discriminates porcine T helper cells with functionally distinct properties.
    Vet Res. 44: 18.
  3. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  4. Franzoni, G. et al. (2013) Assessment of the Phenotype and Functionality of Porcine CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (CSFV) and Virulent CSFV Challenge.
    Clin Vaccine Immunol. 20: 1604-16.
  5. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  6. Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
    Sci Rep. 12 (1): 15630.
  7. Bettin, L. et al. (2023) Co-stimulation by TLR7/8 ligand R848 modulates IFN-γ production of porcine γδ T cells in a microenvironment-dependent manner.
    Dev Comp Immunol. 138: 104543.
  8. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  9. View The Latest Product References
  10. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.

Synonyms
SWC2
UniProt
F1SL30

MCA5973APC

155983 157349 166135 168409 INN1610

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