IgM antibody | IL-A30

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Mouse anti Bovine IgM:HRP

Mouse anti Bovine IgM

Product Type
Monoclonal Antibody
Clone
IL-A30
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2443PA
Datasheet
E
MSDS
0.1 mg loader

MCA2443GA
Datasheet
E F IP R
MSDS
0.1 mg loader

Mouse anti Bovine IgM, clone IL-A30 recognizes bovine IgM, expressed by bovine B-cells. Clone IL-A30 reacts with both serum IgM and cell surface IgM expressed by B lymphocytes. Mouse anti Bovine IgM, clone IL-A30 recognizes a monomorphic epitope within bovine IgM (Naessens et al.1988).

Studies using clone IL-A30 have demonstrated that the percentage of IgM+ve lymphocytes may vary widely ,ranging between 4 - 30%, within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals (Naessens et al.1988).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine peripheral blood mononuclear cells depleted of T lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA 1/500 1/5000
Flow Cytometry 1/25 1/50
Immunoprecipitation
Radioimmunoassays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
Mouse anti Bovine IgA MCA2438GA E IP 0.1 mg loader
Mouse anti Bovine IgA:HRP MCA2438P E 0.25 mg loader
Mouse anti Bovine IgG MCA2439GA E IP 0.1 mg loader
Mouse anti Bovine IgG:HRP MCA2439P E 0.25 mg loader
Mouse anti Bovine IgG1 MCA2440GA C E F IP 0.1 mg loader
Mouse anti Bovine IgG1:HRP MCA2440P E 0.25 mg loader
Mouse anti Bovine IgG2 MCA2441GA E F IP 0.1 mg loader
Mouse anti Bovine IgG2:HRP MCA2441P E 0.25 mg loader

Product Specific References

References for IgM antibody

  1. Naessens, J. et al. (1988) Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.
    Immunology. 63 (4): 569-74.
  2. Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
    Vet Immunol Immunopathol. 24 (3): 267-83.
  3. Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
    J Leukoc Biol. 63 (5): 567-74.
  4. Menge, C. et al. (2004) Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
    Infect Immun. 72 (4): 1896-905.
  5. Naessens, J. (1997) Surface Ig on B lymphocytes from cattle and sheep.
    Int Immunol. 9 (3): 349-54.
  6. Morrison, W.I. et al. (1996) Pathogenicity of Theileria parva is influenced by the host cell type infected by the parasite.
    Infect Immun. 64 (2): 557-62.
  7. Nishimori, A. et al. (2016) Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection.
    J Vet Med Sci. 78 (5): 791-6.
  8. Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
    PLoS One. 11 (3): e0152192.
  9. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.