IgM antibody | IL-A30
Mouse anti Bovine IgM, clone IL-A30 recognizes bovine IgM, expressed by bovine B-cells. Clone IL-A30 reacts with both serum IgM and cell surface IgM expressed by B lymphocytes. Mouse anti Bovine IgM, clone IL-A30 recognizes a monomorphic epitope within bovine IgM (Naessens et al.1988).
Studies using clone IL-A30 have demonstrated that the percentage of IgM+ve lymphocytes may vary widely ,ranging between 4 - 30%, within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals (Naessens et al.1988).
Studies using clone IL-A30 have demonstrated that the percentage of IgM+ve lymphocytes may vary widely ,ranging between 4 - 30%, within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals (Naessens et al.1988).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- HRP Stabiliser (BUF052A)
0.01% Thiomersal - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine peripheral blood mononuclear cells depleted of T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA | 1/500 | 1/5000 | |
| Flow Cytometry | 1/25 | 1/50 | |
| Immunoprecipitation | |||
| Radioimmunoassays |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| Mouse anti Bovine IgA | MCA2438GA | E IP | 0.1 mg |
|
|
| Mouse anti Bovine IgA:HRP | MCA2438P | E | 0.25 mg |
|
|
| Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG:HRP | MCA2439P | E | 0.25 mg |
|
|
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG1:HRP | MCA2440P | E | 0.25 mg |
|
|
| Mouse anti Bovine IgG2 | MCA2441GA | E F IP | 0.1 mg |
|
|
| Mouse anti Bovine IgG2:HRP | MCA2441P | E | 0.25 mg |
|
Product Specific References
References for IgM antibody
-
Naessens, J. et al. (1988) Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.
Immunology. 63 (4): 569-74. -
Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
Vet Immunol Immunopathol. 24 (3): 267-83. -
Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
J Leukoc Biol. 63 (5): 567-74. -
Menge, C. et al. (2004) Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
Infect Immun. 72 (4): 1896-905. -
Naessens, J. (1997) Surface Ig on B lymphocytes from cattle and sheep.
Int Immunol. 9 (3): 349-54. -
Morrison, W.I. et al. (1996) Pathogenicity of Theileria parva is influenced by the host cell type infected by the parasite.
Infect Immun. 64 (2): 557-62. -
Nishimori, A. et al. (2016) Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection.
J Vet Med Sci. 78 (5): 791-6. -
Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS One. 11 (3): e0152192. -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571.
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