Antibodies for the analysis of signal transduction pathways
Cell signaling is a complex process that enables communication between cells and the transduction of a signal from the cell surface all the way to the nucleus. In order to efficiently transmit the signal a myriad of different proteins are required ranging from growth factors/ligands that bind to cell surface receptors to transcription factors altering gene expression patterns. Changes in the levels of biochemical molecules, like nitric oxide and cyclic GMP, and modifications, such as phosphorylation, ubiquitination, neddylation, and sumoylation, are the main ways to convey these signals.
Extensive research has enabled the elucidation of many cell signaling mechanisms and has resulted in the mapping of entire cell signaling pathways. These pathways, which are often named after their main kinase protagonists (e.g. MAP kinase pathway, PI3K-Akt pathway), feature sophisticated feedback loops and due to extensive cross-talk should be viewed as part of a larger network rather than a simplistic single pathway. Misregulation of cell signaling pathways has been implicated in a variety of diseases including cancer, type II diabetes and Parkinson’s disease. Based on this it is not surprising that a lot of effort into developing targeted inhibitors against specific cell signaling molecules or protein interactions.
For your cell signaling research, Bio-Rad offers antibodies for the characterization of kinases, phosphatases, phosphorylated proteins, SUMO mediated signaling, transcription factors and ubiquitin mediated signaling by ELISA, flow cytometry, immunofluorescence, immunohistochemistry, immunoprecipitation and western blotting.
Read more on how to select a good antibody for your application.
Staining of formalin fixed,
human ductal and lobular epithelium with
Rabbit Anti-Focal Adhesion Kinase (AHP1106).
Focal Adhesion Kinase (FAK), also known as PTK2 protein tyrosine kinase 2 (PTK2), is a non-receptor tyrosine kinase that plays an essential role in regulating cellular processes such as cell migration, spreading and cell adhesion. Its hypothesized involvement in cancer cell proliferation and well documented interactions with proto-oncogenes (e.g. EGF R, VEGFR) and tumor suppressor proteins (e.g. p53) make it an ideal target for the development of novel cancer treatments.
Rabbit Anti-FAK Antibody (AHP1106) has been tested in Immunohistochemistry-paraffin and detects an epitope within the internal region of FAK.
Related Products: Cell proliferation reagents, Antibodies for the characterization of oncoproteins, Antibodies for the analysis of tumor suppressor proteins
Western blot analysis of whole cell lysates probed with Anti-MEK 1 Antibody (VMA00010K) followed by detection with HRP conjugated Goat Anti-Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to MEK 1 (molecular weight 42 kDa).
The Anti-MEK1 Antibody (VMA00010K) is part of the PrecisionAb range and has been extensively validated in Western Blotting.
PrecisionAb products are characterized by their high specificity, high sensitivity and batch to batch consistency (every lot of antibody meets the same strict quality control criteria).
For more information, check our PrecisionAb range.
Western blot analysis of whole cell lysates probed with Mouse Anti-ROCK1 Antibody (VMA00614) followed by detection with HRP conjugated Goat Anti-Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to ROCK1 (molecular weight 185 kDa).
ROCK1 is a serine⁄threonine kinase that acts as a downstream effector of Rho. It is a key regulator of the actin cytoskeleton, cell polarity and cancer cell invasion. Activation of ROCK has been implicated in tumor formation in mice due to its importance in the maintenance of actomyosin contractility and cell proliferation regulation. Strong evidence also suggests a role for ROCK in glucose metabolism. Major downstream ROCK1 substrates include DAPK3, FHOD1, GFAP, JIP3, LIMK1, LIMK2, MLC, PFN1, PPP1R12A. ROCK-1 is a direct cleavage substrate of activated caspase-3.
Applications: Western Blotting
Related Products: Antibodies for kinase research
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