Antibodies for Kinases

Kinases

Antibodies for the analysis of kinases

Molecular model of the MEK kinase inhibitor drug Trametinib

Molecular model of the MEK kinase inhibitor drug Trametinib, which is used for the treatment of metastatic melanoma.

During a phosphorylation event, a phosphate group becomes added by a kinase to a serine, threonine or tyrosine residue of a target protein or substrate. This process can be reversed by another group of enzymes called phosphatases . Both kinases and phosphatases are specific with regards to whether they are phosphorylating or dephosphorylating tyrosine or serine/threonine residues.

Phosphorylation is a tightly regulated and fairly ubiquitous cellular process (according to Onclive.com “Today, it is believed that as many as one-third of all proteins in the cell are phosphorylated at one time or another, and half of these proteins likely harbor more than 1 phosphorylation site, with different sites often eliciting quite different cellular responses.” (De Lartigue J 2011)).

The process is also critical as aberrant phosphorylation levels and misregulation of phosphorylation cascades have been implicated in a variety of diseases, including cancer. Due to this correlation, much focus has recently been placed on developing specific kinase inhibitors as potential cancer treatments. One of these inhibitors currently used for the treatment of colorectal and head and neck cancers is Cetuximab , a monoclonal antibody, which inhibits EGF receptors.


Antibodies for Kinase Research

Bio-Rad offers a variety of antibodies for analyzing kinases by ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blotting. For your convenience, we have grouped our research reagents into the main seven kinase families:

  • AGC - family members include PKA, PKC and PKG (Martin et al. 2008)
  • CAMK  - includes calmodulin/calcium regulated kinases (Martin et al. 2008)
  • CMGC   - this class includes MAPK, CDK and GSK3 kinases (Martin et al. 2008)
  • STE - many of the STE kinases are involved in MAP kinase cascade signaling (Martin et al. 2008)
  • TK - family members include receptor tyrosine kinases such as the EGF receptor (Martin et al. 2008)
  • TKL  - tyrosine like kinases are serine/threonine kinases that have a close sequence similarity to tyrosine kinases; family members include mixed lineage kinases (Martin et al. 2008)
  • Other kinases

AGC

CAMK

CMGC

STE

TK

TKL

Other

AKT1
AKT2
Protein kinase C
ROCK2
SGK3
STK38
AMPK
CAMK
Chk1
Chk2
Zip
Cdk2
Cdk5
Cdk7
Cdk11
Erk/MAPK
Jnk / MAPK8
p38 / MAPK
Cdk4
Cdk6
Cdk9
Erk1
GSK3
Jnk3
ASK1
MAPK
MEK1
MEK1/2
MEK5
MKNK2
PAK1
PAK2
PAK3
PAK4
PAK6
CD246 / ALK
AXL
c-Met
CSK
c-Src
EGFR
ErbB3
ErbB4
Focal adhesion kinase
Fyn
HER2/neu (Erb2)
LCK
Lyn
Syk
ALK7
ILK1
IRAK1
IRAK2
IRAK3
IRAK4
M3K11
VEGFR1
VEGFR2
VEGFR3
WNK1
Zap70
Aurora A kinase
Aurora B kinase
IKK
Nemo like kinase
PINK
PLK1
PLK3
ULK2
WEE1

Antibodies for Kinases Research

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    References 

    De Lartigue J (2011). Phosphorylation: The Master Switch of the Cell. http://www.onclive.com/publications/Oncology-live/2011/november-2011/Phosphorylation-The-Master-Switch-of-the-Cell, accessed June 29, 2015.

    Martin et al. (2008). Kinomer v. 1.0: a database of systematically classified eukaryotic protein kinases Nucleic Acids Research, doi: 10.1093/nar/gkn834.