Mastering IHC staining experiments


Mastering IHC staining experiments

In an Immunohistochemistry (IHC) experiment, a primary antibody binds specifically to a protein of interest present in a tissue. The antibody binding is then visualized by a detection system, which provides information about if and where the protein is present in the tissue. IHC is a common method in life sciences to determine morphological abnormalities and the presence of markers indicative of certain diseases, such as cancer.

Featured rabbit anti-FAK antibody (AHP1106)

Staining of formalin fixed, paraffin embedded human ductal and lobular epithelium with rabbit anti-Focal Adhesion Kinase (AHP1106).

The "Mastering Immunohistochemistry staining experiments" webinar gives you a basic overview of the different IHC protocol steps (with a focus on sample preparation, reagent selection, antigen retrieval and antibody staining). We discuss the most common pitfalls and provide hands-on tips on how to troubleshoot this complex type of experiment. We recommend this webinar to IHC novices and researchers with limited experience of this technique.

Topics include:

  • IHC procedure overview
  • Most common IHC pitfalls 
  • Tips and tricks and troubleshooting advice
  • IHC protocol steps (with a focus on sample preparation, reagent selection, antigen
    retrieval and antibody staining)

Webinar: Mastering IHC experiments

Presented by: Dr Judith Langenick (Product Manager at Bio-Rad)

Speaker Biography: Dr Judith Langenick (Product Manager at Bio-Rad)

Judith graduated from the University of Dundee (Scotland) with a B.Sc. in Molecular Biology and a Ph.D. Her thesis research, in the group of Prof. Jeffrey Williams, focused on elucidating STAT signaling pathways in the social amoeba Dictyostelium discoideum. For her postdoctoral research, Judith moved to Dr. Ketan J. Patel’s group at the MRC Laboratory of Molecular Biology in Cambridge, where she was investigating the role of the Fanconi Anemia DNA Repair Pathway. Since leaving academic research Judith has held product management positions in both Germany and the United Kingdom during which she was involved in the development and global launch of novel antibody portfolios.

For more information about Immunohistochemistry, please refer to our IHC resources: