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Tidy up your IP/Western blot with one simple reagent change
Generate publication quality Western blots
Loading Control Antibodies - Generate publication quality Western blots
A Western blot provides a readout of differences in protein expression levels e.g. changes in protein levels over a certain time period, differences in knock-out versus wild type cell lines and altered expression in patient samples. However, in order to draw any conclusions from a Western blot one has to be sure that the observed differences are only due to altered protein expression levels rather than gel loading or protein transfer errors. For this purpose Western blot loading controls are used.
She, X, et al., (2009) Definition, conservation and epigenetics of housekeeping and tissue-enriched genes.
BMC Genomics 10:269.
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