Unlike secondary antibodies, TidyBlot Reagent specifically binds to native (non-reduced) antibodies, enabling the detection of immunoblotted target protein bands, without the interference from denatured IgG.
Sources of denatured IgG include endogenous IgGs present in cell/tissue lysates (for instance in spleen and liver samples;
problem 1) and primary antibodies released from beads during immunoprecipitation experiments (problem 2). The latter are particularly obstructive when western blot detection of (co-)immunoprecipitated proteins is desired, as IgG heavy (~50 kDa) and light chains (~25 kDa) may mask the protein(s) of interest.
Detects only what matters – TidyBlot exclusively binds to native non-denatured antibodies and not to any IgGs present in your immunoprecipitate or lysate. Avoids obstruction by IgG heavy and light chains and produces background free western blots
Convenience – just substitute your conventional secondary antibody for TidyBlot
Versatility and broad species coverage – detects a variety of monoclonal and polyclonal antibodies. No need to buy several HRP conjugated secondary antibodies