Vedolizumab antibody | AbD30136

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Human anti Vedolizumab

Human Anti-Vedolizumab Antibody is a recombinant, inhibitory anti-idiotypic antibody in monovalent Fab format; it is recommended as capture antibody in a PK bridging ELISA format with HCA294P for detection.

Product Type
Monoclonal Antibody
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Human Anti-Vedolizumab Antibody, clone AbD30136 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug vedolizumab. The antibody does not recognize free recombinant human α4β7 integrin or vedolizumab in complex with α4β7 integrin and can be used to measure free vedolizumab levels in serum from patients.

A pair of anti-vedolizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an anti-vedolizumab antibody in full immunoglobulin format, clone AbD30138_hIgG1 (HCA294) as the detection antibody.

Vedolizumab (Entyvio) is a humanized antibody (IgG1/kappa), derived from a murine antibody. It is used for the treatment of both moderate to severe ulcerative colitis and moderate to severe Crohn's disease, primarily in patients who have lost response to, or are intolerant to inhibitors of tumor necrosis factor alpha (TNFα), such as infliximab, adalimumab. The drug binds specifically to human α4β7 integrin, a key mediator of gastrointestinal inflammation expressed on the surfaces of T and B lymphocytes. By selectively inhibiting the binding of α4β7 integrin to the mucosal addressin cell adhesion molecule-1 (MAdCAM-1), vedolizumab prevents leukocyte binding to the endothelial surface and extravasation into affected tissue. Specifically inhibiting this pathway alleviates gastrointestinal inflammation without impairing systemic immune responses.

View a summary of all anti-vedolizumab antibodies

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
The monovalent intrinsic affinity of AbD30136 was measured as KD=0.1 nM by real time, label-free molecular interaction analysis on immobilized vedolizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Entyvio is a trademark of Millennium Pharmaceuticals, Inc.
His-tag is a trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
For research purposes only

Applications of Vedolizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Clone AbD30136 can be used in a direct or indirect ELISA or as capture antibody for vedolizumab in a bridging ELISA together with (HCA293, HCA294, HCA294P, HCA295) as the detection reagent.

Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rat anti DYKDDDDK Tag MCA4764 E F IF IP P WB 0.2 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
HuCAL Fab-FH Negative Control HCA045 E 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Vedolizumab HCA293 E 0.1 mg loader
Human anti Vedolizumab HCA294 E 0.1 mg loader
Human anti Vedolizumab:HRP HCA294P E 0.1 mg loader
Human anti Vedolizumab HCA295 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody

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