T Lymphocytes Antibody | KEN-5

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T Lymphocytes Antibody | KEN-5 gallery image 1

Staining of rabbit peripheral blood lymphocytes with Mouse anti Rabbit T Lymphocytes (MCA800G)

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Staining of rabbit peripheral blood lymphocytes with Mouse anti Rabbit T Lymphocytes:FITC (MCA800F)

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T Lymphocytes Antibody | KEN-5 gallery image 3

Staning of rabbit peripheral lymphocytes with Mouse anti Rabbit T Lymphocytes (MCA800GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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  • Product Type
    Monoclonal Antibody
  • Clone
    KEN-5
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA800GAC, E, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA800GA
    MCA800FFdatasheet pdfdatasheet pdf100 Tests
    MCA800F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rabbit T lymphocytes, clone KEN-5, recognizes rabbit (lapine) T-cells. Clone KEN-5 recognizes >90% thymocytes and approximately 40-45% mesenteric lymph node cells and immunoprecipitates a 67 kDa protein from rabbit thymocytes.

      In contrast to humans and mice where only a very small population of B-cells express the CD5 antigen, the CD5 antigen in rabbits is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognising rabbit CD5 (Kotani et al.1993), does not bind to rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognising rabbit CD5 and anti human cross reactive CD5 antibodies, shows very limited binding to rabbit B-cells, which in adult rabbits have been demonstrated to express CD5 (Raman & Knight 1992) More recently studies (Pospisil et al. 2009) indicate that clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. It has therefore been suggested that KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells (Pospisil et al. 2009).
    • Intended Use
    • Target Species
      Rabbit
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Rabbit Thymocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the PAI mouse myeloma cell line
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/200
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional T Lymphocytes Antibody Formats

    Formats Clone Applications Sizes available
    T Lymphocytes Antibody : FITC KEN-5 F 100 Tests
    T Lymphocytes Antibody : Purified KEN-5 C, E, F, IP 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen

              References

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