CD3 antibody | CD3-12
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used for the detection of infiltrating T cells in formalin fixed, paraffin embedded kidney tissue sections.
Image caption:
Immunohistochemistry for inflammatory cells in kidneys of normal control mice, vehicle-treated mice with anti-GBM-GN, and EGCG-treated mice with anti-GBM-GN. Kidneys from mice 4 weeks after induction of anti-GBM-GN with or without EGCG treatment and kidneys from normal control mice were evaluated for inflammatory cell infiltration. Immunohistochemical analyses showed markedly increased interstitial infiltration of lymphocytes and macrophages in the vehicle-treated group (original magnification x 400). In contrast, the EGCG-treated animals exhibited significantly less interstitial infiltration of lymphocytes and macrophages. No significant inflammatory cell infiltrates were observed in the normal control mice. The number of lymphocyte and macrophages in glomeruli and interstitial compartment are depicted in the bar graphs. Values are mean ± SEM; n = 8–10 in each group.
From: Ye T, Zhen J, Du Y, Zhou JK, Peng A, et al. (2015) Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate Restores Nrf2 Activity and Ameliorates Crescentic Glomerulonephritis.
PLoS ONE 10(3): e0119543.
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used for the detection of CD3 expressing cells in murine tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Insert image caption
From: Flatz L, Rieger T, Merkler D, Bergthaler A, Regen T, et al. (2010) T Cell-Dependence of Lassa Fever Pathogenesis.
PLoS Pathog 6(3): e1000836.
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone CD3-12 (MCA1477) used for the detection of CD3 expressing cells in human spleen by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Spectral imaging analysis of human spleen stained for CD3 (Warp Red), CD37 (True Blue) and cell nuclei (Nuclear Red). a Representative original multispectral image. Scale bar = 100 μm. Composite RGB image (b) of unmixed CD3 (c in red), CD37 (d in blue) and Nuclear Red (e in green in composite RGB image) signal using the spectral library (Fig. 4a). f Tissue segmentation; B cell follicle (B, yellow), T cell zone (T, red), red pulp (RP, green) and other tissue (blood vessels, collagen; blue). g Segmentation of individual cells (green) based on Nuclear Red staining. h Thresholds for Warp Red and True Blue staining were set to score CD3−CD37dim (blue), CD3+CD37dim (red), CD3+CD37bright (yellow) or CD3−CD37bright (green) cells. i–l Scatter plots showing optical densities for CD3 (Y-axis) and CD37 (X-axis) of individual cells in T cell zones (i, l green), B cell follicles (j, l blue) and red pulp regions (k–l red) and thresholds used for scoring (dotted lines). A representative of 2000 cells per tissue region is plotted
From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015 May 8. [Epub ahead of print]
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD3 antibody, clone CD3-12 (MCA1477) used for the detection of CD3 expressing cells in human bone marrow, spleen and appendix by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Localization and expression of CD37 in bone marrow (a–f), spleen (g–l) and appendix (m–r). a, g, m Representative original multispectral image of lymphoid organ stained for CD3 (Warp Red), CD37 (True Blue) and cell nuclei (Nuclear Red). Scale bars = 100 μm. b, h, n Composite RGB image after spectral unmixing of original image (red CD3, blue CD37, green nuclei). c, i, o Image showing scoring of CD3−CD37dim (blue), CD3+CD37dim (red), CD3+CD37bright (yellow) or CD3−CD37bright (green) cells. Optical density of CD37 on individual cells in human bone marrow (d), in B cell follicle (blue line) and in T cell zone and in red pulp (red line) in human spleen (j) and in B cell follicle (blue line) and in lamina propria (red line) in human appendix (p). Optical densities were binned per 0.05 and normalized to % of max. Percentage of CD37dim and CD37bright cells in total bone marrow (e) and in the CD3+ cell population in human bone marrow (f). Percentage of total CD37bright cells in B cell follicle and red pulp (k) and in the CD3+ cell population in T cell zone and red pulp (l) in human spleen. Percentage of total CD37bright cells (q) and in the CD3+ cell population (r) in B cell follicle and lamina propria in human appendix. Each dot represents data of one ×20 image from the lymphoid tissue. The red line represents the mean. *P<0.05, ****P<0.0001
From: de Winde CM, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor CG, van Spriel AB.
Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
Histochem Cell Biol. 2015 May 8. [Epub ahead of print]
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used for the detection of T lymphocytes in mouse nasal associated lymphoid tissue by immunofluorescence.
Image caption:
Population of 2W:I-Ab tetramer+ cells detected in situ in NALT co-stained with CD3 but not IgG antibodies. (A) Representative image of a whole NALT section from a GAS-2W infected mouse stained with 2W:I-Ab tetramers (red) with one round of TSA amplification, CD3 antibodies (blue), and IgG antibodies (green). For this image, several confocal z-scan fields were collected using a 20X objective and reconstructed as a montage. The enlargement in (B) shows a 2W:I-Ab tetramer-binding T cell that is co-stained with CD3 antibodies but not IgG antibodies. The enlargement in (C) shows 2W:I-Ab tetramer-binding cells that are not co-stained with CD3 or IgG antibodies. (B) and (C) are confocal z-scans collected using the same parameters with a 60X objective and zoom of 3. 2W:I-Ab tetramer+CD3+IgG- cells are indicated with arrowheads, and 2W:I-Ab tetramer+CD3-IgG- cells are indicated with arrows.
From: Dileepan T, Kim HO, Cleary PP, Skinner PJ (2015)
In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues. PLoS ONE 10(6): e0128862.
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Human CD3&epsilon antibody, clone CD3-12 (MCA1477) used to identify CD3&epsilon expressing cell by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
CD3+ cells in the lungs following primary infection and reinfection.
Immunofluorescence (IF) image of CD3 (green) and virus antigen (red) within the same perivascular region following reinfection (C). IF images at 40x, bar equivalent to 20μm.
From: Houser KV, Broadbent AJ, Gretebeck L, Vogel L, Lamirande EW, Sutton T, et al. (2017)
Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody.
PLoS Pathog 13(8): e1006565.
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to illustrate CD3 positive cells at the peak of the inflammatory response in ears of imiquimod treated mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Characterization of the inflammatory infiltrate in IMQ-treated ears.
Representative anti-Ly6G, anti-CD3 stained sections are shown for IMQ-treated WT ears at the peak of inflammation on day 7. Representative anti-Ly6G (brown staining, left panels) and anti-CD3 (brown staining, right panels) stained sections are shown for IMQ-treated ears of WT (upper panels) or Il-38-/- (lower panels) littermate mice at the peak of inflammation on day 7 (C). Scale bar = 100 μm.
From: Palomo J, Troccaz S, Talabot-Ayer D, Rodriguez E, Palmer G (2018)
The severity of imiquimod-induced mouse skin inflammation is independent of endogenous IL-38 expression.
PLoS ONE 13(3): e0194667.
This is an open access article distributed under the terms of a Creative Commons Attribution License.
AlexaFluor® 647 conjugated Rat anti Human CD3 antibody, clone CD3-12 (MCA1477A647) used for identifying CD3 expressing lymphocytes by flow cytometry.
Image caption:
Example gating scheme for identification of CD3+/CD4+ lymphocytes and CD21+ lymphocytes. Lymphocytes were identified and gated using a forward and side scatter plot (a). Gated lymphocytes were applied were then applied to PE (CD3) vs FITC (CD4) plots or an Alexa Fluor 647 (CD21) histogram, respectively. Unstained cells and matched isotype controls from the same manufacturer were used to determine cut offs for negative (b and d) versus positive (c and e) cells. CD3+/CD4+ or CD21 positive cells were then identified. CD3+/CD8+ cells were selected in the same manner as the CD3+/CD4+ cells
From: DeClue AE, Axiak-Bechtel SM, Zhang Y, Saha S, Zhang L, Tung DD, Bryan JN.
Identification of immunologic and clinical characteristics that predict
inflammatory response to C. Novyi-NT bacteriolytic immunotherapy.
BMC Vet Res. 2018 Apr 2;14(1):119.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used for the demonstration of T-cell infiltrates in murine kidney sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Tyrphostin AG490 reduced inflammation and tubulointerstitial injury in obstructed kidneys after unilateral ureteral obstruction (UUO) in neonatal mice.
Mice received Tyrphostin AG490 or vehicle by daily injections. Renal sections of UUO- and sham-operated kidneys were stained for T-cell infiltration (CD-3 antibody) at 3, 7, and 14 days of life and analyzed in 20 high-power fields (hpf) per section at x400. Bar = 100 μm. Data are the mean ± s.e. (n = 10 in each group).
From: Gasparitsch M, Schieber A, Schaubeck T, Keller U, Cattaruzza M, Lange-Sperandio B (2019)
Tyrphostin AG490 reduces inflammation and fibrosis in neonatal obstructive nephropathy.
PLoS ONE 14(12): e0226675.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 (MCA1477) used to label T cells in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical stains and quantification of samples for each genotype. CD3-positive T cell. No statistically significant changes were found in either analysis. Scale bar = 100 μm.
From: Datta, M.; Hansen, S.M.; Staszewski, O.
Microglial Expression of Hdac1 and Hdac2 is Dispensable for Experimental Autoimmune Encephalomyelitis (EAE) Progression.
J 2020, 3, 358-65.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 (MCA1477) used to label T cells in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative histological and immunohistochemical stainings of (a) spinal cord lesions
(d) T-cell infiltrates (CD3), Scale bar = 500 μm.
From: Datta, M.; Hansen, S.M.; Staszewski, O.
Microglial Expression of Hdac1 and Hdac2 is Dispensable for Experimental Autoimmune Encephalomyelitis (EAE) Progression.
J 2020, 3, 358-65.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to demonstrate T-cell infiltrates in murine aortic arches by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
The accumulation of CD3+ T cells (c) into ain murine aortic arches by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.ortic tissues in WT and Ccl3−/− mice.
Representative results from six independent experiments. Scale bar, 50 μm.
From: Ishida Y, Kuninaka Y, Nosaka M, Kimura A, Taruya A, Furuta M, Mukaida N, Kondo T.
Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis.
Nat Commun. 2020 Nov 25;11(1):5994.
doi: 10.1038/s41467-020-19763-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to stain T-cells in murine brain by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Segregation of the meningeal lymphocyte niche into the anatomical layers.
(A) Representative scanning electron microscopy (SEM) images showing separation of the three spinal cord meningeal layers. (B) Representative immunofluorescent whole-mount staining of the spinal cord pia and dura meningeal layers, for T cells and B cells under uninjured conditions. Scale bar: 20 μm. (C, D) Representative immunofluorescent whole-mount staining of the pia (C), and dura (D) meningeal B- and T-cell clusters at d 14 after SCI. Scale bar; 50 μm. (E) Whole-mount quantification of the number of B cells (black) or T cells (white) in each cluster present at the pia and dura layers of uninjured mice, and along different time points following SCI. (E) Results are pooled from three animals at each time point, and normalized to the indicated area.
From: Cohen M, Giladi A, Raposo C, Zada M, Li B, Ruckh J, Deczkowska A, Mohar B, Shechter R, Lichtenstein RG, Amit I, Schwartz M.
Meningeal lymphoid structures are activated under acute and chronic spinal cord pathologies.
Life Sci Alliance. 2020 Dec 4;4(1):e202000907.
doi: 10.26508/lsa.202000907.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to label SIV infected, African greem monkey lymph nodes by immunofluorescence.
Image caption:
Confocal images of LN from MAC and AGM collected in acute or chronic infection. Representative examples are shown for one animal among four studied. Pseudocolors were attributed to each marker (blue for DAPI, green for MHC-E, red for CD3 or SIV RNA, as indicated on the figure). Scale bars are shown on the figure.
B cell follicles co-stained for MHC-E and CD3.
From: Huot N, Rascle P, Petitdemange C, Contreras V, Stürzel CM, Baquero E, Harper JL, Passaes C, Legendre R, Varet H, Madec Y, Sauermann U, Stahl-Hennig C, Nattermann J, Saez-Cirion A, Le Grand R, Keith Reeves R, Paiardini M, Kirchhoff F, Jacquelin B, Müller-Trutwin M.
SIV-induced terminally differentiated adaptive NK cells in lymph nodes associated with enhanced MHC-E restricted activity.
Nat Commun. 2021 Feb 24;12(1):1282.
doi: 10.1038/s41467-021-21402-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to label T lymphocytes in rabbit muscle by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Image sections of immunohistochemically stained cranial tibial muscle cross sections. (A) Periimplant fibrous tissue of an MgCa0.8 screw six weeks post operatively (CD79α, 400×). Arrows represent CD79α positive B-lymphocytes. (B) Periimplant fibrous tissue of an S316L screw two weeks postoperatively (CD3, 400×). Triangles indicate CD3 positive T-lymphocytes.
From: Erdmann N, Bondarenko A, Hewicker-Trautwein M, Angrisani N, Reifenrath J, Lucas A, Meyer-Lindenberg A.
Evaluation of the soft tissue biocompatibility of MgCa0.8 and surgical steel 316L in vivo: a comparative study in rabbits.
Biomed Eng Online. 2010 Oct 25;9:63.
doi: 10.1186/1475-925X-9-63.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to label T-cells in murine liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Protective Activity of Ruxolitinib is not Linked to iNOS Expression, Serum NOx Levels or Viral Load. HHD mice were infected with LCMV on day 0 and treated with Ruxolitinib or anti-TNF.
8 days post infection, liver tissues were prepared for histological analysis and were compared to uninfected HHD mice. Sections were processed for immunohistochemical detection of T cells (CD3). Scale bar: 100 μm. Scale bar in insets: 20 μm.
From: Sahin M, Remy MM, Merkler D, Pinschewer DD.
The Janus Kinase Inhibitor Ruxolitinib Prevents Terminal Shock in a Mouse Model of Arenavirus Hemorrhagic Fever.
Microorganisms. 2021; 9(3):564.
doi: 10.3390/microorganisms9030564
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to label T-lymphocytes in murine spleen by Immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Histological analysis of the spleen (a) of 2 week old mice with a combined Cx3cr1-dependent knockout of Hdac1 and 2 (Cre+) compared to Cre-negative littermates (Cre−). Histomorphology (HE) and cellular composition (macrophages (Mac3), T-lymphocytes (CD3) and B-lymphocytes (B220)) are depicted. Representative images of 5 Cre+ and 5 Cre− animals are shown. Scale bar = 500 μm
From: Datta M, Staszewski O.
Hdac1 and Hdac2 are essential for physiological maturation of a Cx3cr1 expressing subset of T-lymphocytes.
BMC Res Notes. 2021 Apr 13;14(1):135.
doi: 10.1186/s13104-021-05551-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to label T-lymphocytes in murine spleen by Immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
a Mice with combined knockout of Hdac1 and 2 (Cre+) show markedly reduced life span compared to wildtype littermates (Cre−). b At 16 weeks of age thymus, spleen and lymph nodes are strongly enlarged in knockout (Cre+) animals when compared to wildtype littermates (Cre−). c Histology and immunohistochemistry of 16 week old mice, comparing spleen samples of mice with combined Cx3cr1-dependent knockout of Hdac1 and 2 (Cre+) to their wildtype littermate (Cre−). Macrophages (Mac3), T-lymphocytes (CD3) and B-lymphocytes (B220) are depicted. Representative pictures of 5 Cre+ and 5 Cre−. Scale bar = 500 μm.
From: Datta M, Staszewski O.
Hdac1 and Hdac2 are essential for physiological maturation of a Cx3cr1 expressing subset of T-lymphocytes.
BMC Res Notes. 2021 Apr 13;14(1):135.
doi: 10.1186/s13104-021-05551-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to identify CD3ε expressing cells by immunohistochemistry on formalin fixed, paraffin embedded rabbit tissue sections.
Image caption:
CD3+ cells in the lungs following primary infection and reinfection.
DAB images from primary infection (A) and reinfection (B). DAB images from day 3 post-infection at 10x, bar equivalent to 100μm.
From: Houser KV, Broadbent AJ, Gretebeck L, Vogel L, Lamirande EW, Sutton T, et al. (2017)
Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody.
PLoS Pathog 13(8): e1006565.
This is an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Human CD3, clone CD3-12 (MCA1477) used to detect murine T cells in brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Inflammation and immune cell infiltration in the cerebellum at day 62 p.i.
Immune cell infiltration in cerebellar WML at day 62 p.i. Fgfr1ind−/− mice showed a decreased number of CD3(+) T cells (F–H) in WML of the cerebellum. n = 3/group. Scale bar depicts 50 μm (F–P). Data are expressed as the mean ± SEM. * p <0.05, ** p <0.01. *** p <0.001.
From: Rajendran R, Rajendran V, Giraldo-Velasquez M, Megalofonou FF, Gurski F, Stadelmann C, Karnati S, Berghoff M.
Oligodendrocyte-Specific Deletion of FGFR1 Reduces Cerebellar Inflammation and Neurodegeneration in MOG35-55-Induced EAE.
Int J Mol Sci. 2021 Aug 31;22(17):9495.
doi: 10.3390/ijms22179495.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to identify T-lymphocytes in cynomolgus monkey liver by immunofluorescence.
Image caption:
Immunofluorescence detection of CD3+ and CD8+ lymphocytes at 67 dpi in liver sections of HEV infected cynomolgus monkey.
Detection of CD8+ cells (white arrow) and CD8+ T lymphocytes (CD3+CD8+) (yellow arrow) by IFI. (A) CD8 T lymphocytes (I3—Swine HEV-3); (B-D) CD8+ cells and T CD8 lymphocytes (X15, G3 e F3—Swine HEV-3); (E) T CD4 lymphocytes (J3—Human HEV-3); (F) CD8+ cell (I2—Control). The monkeys’s identification is shown on the bottom left- hand side. Anti-CD3/Alexa fluor 647 (red), Anti-CD8/FITC (green), co-staining CD3+CD8+ (yellow—regions of overlap) and Nuclei stained with DAPI (blue) and counterstained with Evans blue (red). (G) Frequency of CD8+ cells and CD8 (CD3+CD8+) T lymphocytes in hepatic parenchyma from experimental groups (Swine HEV-3 [n = 6], Human HEV-3 [n = 2] and Control [n = 2]). Bars represent the means with the standard error. Selections with * (0.01 ≤ p <0.05), ** (0.001≤ p <0.01) and *** (p <0.001) represent significant differences between the groups. Results analyzed with non-parametric Kruskal-Wallis and Dunn’s Multiple Comparison test. HPF, high-power field.
From: Mejido DCP, de Oliveira JM, Gaspar AMC, Gardinali NR, Bottino FdO, de Carvalho LG, et al. (2019)
Evidences of HEV genotype 3 persistence and reactivity in liver parenchyma from experimentally infected cynomolgus monkeys (Macaca fascicularis).
PLoS ONE 14(6): e0218472.
doi: 10.1371/journal.pone.0218472
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to identify T lymphocytes in murine hepatic tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Protective Activity of Ruxolitinib is not Linked to iNOS Expression, Serum NOx Levels or Viral Load. HHD mice were infected with LCMV on day 0 and treated with Ruxolitinib or anti-TNF (D–F) 8 days post infection, liver tissues were prepared for histological analysis and were compared to uninfected HHD mice. Sections were processed for immunohistochemical detection of LCMV NP and T cells (CD3). Scale bar: 100μm. Scale bar in insets: 20μm.
From: Sahin M, Remy MM, Merkler D, Pinschewer DD.
The Janus Kinase Inhibitor Ruxolitinib Prevents Terminal Shock in a Mouse Model of Arenavirus Hemorrhagic Fever.
Microorganisms. 2021 Mar 9;9(3):564.
doi: 10.3390/microorganisms9030564.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to identify T cells in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative immunohistochemistry analysis of CD3+, CD4+, and CD8+ T cells in the cardiac tissues at experimental endpoint. (a) Specific antibody staining of CD3+, CD4+, and CD8+ T cells. In each animal group, the top panels show the whole tissue sections, at 1.5x magnification (long horizontal bar: 2 mm); the bottom panels indicate zoomed sections from rectangular insets in the top panels, at 40x magnification (short horizontal bar: 50 μm). (b) Quantitative analysis of one representative mouse from each group. For consistency, only ventricle areas were evaluated. Predominant inflammatory cells were CD3+ T cells, and CD8+ T cells were higher than CD4+ T cells present in the sections examined.
From: Portillo S, Zepeda BG, Iniguez E, Olivas JJ, Karimi NH, Moreira OC, Marques AF, Michael K, Maldonado RA, Almeida IC.
A prophylactic α-Gal-based glycovaccine effectively protects against murine acute Chagas disease.
NPJ Vaccines. 2019 Mar 22;4:13.
doi: 10.1038/s41541-019-0107-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3 antibody, clone CD3-12 (MCA1477) used to identify infiltrating T-lymphocytes in the murine dorsal root ganglia by immunohistochemistry on cryosections.
Image caption:
Macrophage and T-cell infiltration in DRG of old WT and GLA KO mice. (A–D) Representative photomicrographs of CD11b+ macrophages and CD3+ T-cells (arrowheads) in DRG of old WT ((A,B), respectively) and old GLA KO mice ((C,D), respectively). (E) Quantification of CD11b+ macrophages per mm2 DRG area in old WT (◯, n = 10) and old GLA KO mice (●, n = 10). (F) Quantification of CD3+ T-cells per mm² DRG area in old WT ◯, n = 10) and old GLA KO mice (●, n = 10). Abbreviations: CD = Cluster of Differentiation; DRG = dorsal root ganglia; GLA KO = alpha-galactosidase A knockout; WT = wildtype. Scale bar: 100 μm.
From: Spitzel M, Wagner E, Breyer M, Henniger D, Bayin M, Hofmann L, Mauceri D, Sommer C, Üçeyler N.
Dysregulation of Immune Response Mediators and Pain-Related Ion Channels Is Associated with Pain-like Behavior in the GLA KO Mouse Model of Fabry Disease.
Cells. 2022 May 24;11(11):1730.
doi: 10.3390/cells11111730.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to identify T-cells in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Intravitreal HKMtb generates acute panuveitis that is exacerbated and becomes chronic when preceded by a systemic prime. (J–L) Day 56 PMU retina with DAB+ (J) T cells (CD3+), (K) macrophages and retinal microglia (F4/80+) and (L) neutrophils (Gr-1+). (N–P) Day 56 UMU retina with (N) rare T cells (CD3+), (O) few macrophages and retinal microglia (F4/80+) and (P) no neutrophils (GR-1+).
From: Pepple KL, John S, Wilson L, Wang V, Van Gelder RN.
Systemic prime exacerbates the ocular immune response to heat-killed Mycobacterium tuberculosis.
Exp Eye Res. 2022 Jul 31;223:109198.
doi: 10.1016/j.exer.2022.109198.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human CD3ε antibody, clone CD3-12 (MCA1477) used to identify T-cells in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Additional immunohistochemistry for immune cells in PMU and UMU eyes. (A-F) Day 1 PMU eye stained for T cells with anti-CD3 (A,D), (I–K) Serial sections of a day 56 PMU eyes stained with anti-CD3 (I) (J–R) Day 1 UMU eye stained for T cells with anti-CD3 (J,M),(P–R) Serial sections of a day 56 UMU eyes stained with anti-CD3 (P).
From: Pepple KL, John S, Wilson L, Wang V, Van Gelder RN.
Systemic prime exacerbates the ocular immune response to heat-killed Mycobacterium tuberculosis.
Exp Eye Res. 2022 Jul 31;223:109198.
doi: 10.1016/j.exer.2022.109198.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F P WB IF E C ResetRat anti Human CD3
- Product Type
- Monoclonal Antibody
- Clone
- CD3-12
- Isotype
- IgG1
- Format
- Purified
- Specificity
- CD3
Antibody Quality and Performance Guarantee
| Rat anti Human CD3 antibody, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005). |
|
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Dog Horse Rhesus Monkey Pig Chicken Mouse Duck Koala Harbour Porpoise Alpaca Cynomolgus monkey Spotted Hyena Sea Lion Cat Amazon Parrot Mammals Expected from Sequence Birds Expected from Sequence Raccoon Great horned owl (Bubo virginianus) Bullfrog Xenopus Amphibia Expected from Sequence Rabbit African green monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1477T: Purified IgG prepared from tissue culture supernatant
- MCA1477: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/100 | |
| Immunohistology - Paraffin 2 | |
1/100 | |
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG1 Negative Control | MCA6004GA | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat IgG1 Negative Control | ||||||
References for CD3 antibody
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Holtappels, R. et al. (1998) Control of murine cytomegalovirus in the lungs: relative but not absolute immunodominance of the immediate-early 1 nonapeptide during the antiviral cytolytic T-lymphocyte response in pulmonary infiltrates.
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Hudson, K.J. and Bouton, A.H. (2006) Yersinia pseudotuberculosis adhesins regulate tissue-specific colonization and immune cell localization in a mouse model of systemic infection.
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Tzartos, J.S. et al. (2008) Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis.
Am J Pathol. 172: 146-55.
View The Latest Product References
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Further Reading
-
Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient-derived CD8 T cells.
J Virol. 72 (10): 7733-44. -
Burudi, E.M. et al. (2002) Regulation of indoleamine 2,3-dioxygenase expression in simian immunodeficiency virus-infected monkey brains.
J Virol. 76 (23): 12233-41. -
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- RRID
- AB_321245
- UniProt
- P07766
- Entrez Gene
- CD3E
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0007172 signal complex assembly
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0030159 receptor signaling complex scaffold activity
- View More GO Terms
- GO:0031295 T cell costimulation
- GO:0042608 T cell receptor binding
- GO:0046982 protein heterodimerization activity
- GO:0050852 T cell receptor signaling pathway
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