CD45 antibody | L12/201

Mouse anti Rabbit CD45

Product Type
Monoclonal Antibody
Clone
L12/201
Isotype
IgG1
Specificity
CD45

Product Code Applications Pack Size List Price Your Price Qty
MCA808GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200. Mouse anti Rabbit CD45 antibody, clone L12/201 shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.

Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Glycoproteins isolated from the T cell line, RL-5.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control

References for CD45 antibody

  1. Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
    J Exp Med. 157 (1): 34-46.
  2. Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
    Mol Immunol. 21 (1): 95-103.
  3. Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
    Immunology. 77 (1): 106-8.
  4. Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
    J Pathol. 170 (3): 315-20.
  5. Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
    Transplantation. 89: 409-16.
  6. Mackenzie, S.M. et al. (2006) Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3.
    Biol Reprod. 74: 511-21.
  7. Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
    Arterioscler Thromb Vasc Biol. 21: 220-6.
  8. Liang, H. et al. (2009) Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
    Mol Vis. 15: 1690-9.
  9. View The Latest Product References
  10. Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
    J Leukoc Biol. 85: 44-54.
  11. Kuznetsov, S.A. et al. (2001) Circulating skeletal stem cells.
    J Cell Biol. 153: 1133-40.
  12. Sijnave, D. et al. (2015) Inhibition of Rho-Associated Kinase Prevents Pathological Wound Healing and Neovascularization After Corneal Trauma.
    Cornea. 34 (9): 1120-9.
  13. Kováč, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
    Slovak J Anim Sci., 49,(2): 62–67.
  14. Ondruska, L.. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
    Veterinární Medicína. 61 (No. 10): 571-6.
  15. Davis, W.C. & Hamilton, M.J. (2008) Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules.
    J Vet Sci. 9 (1): 51-66.
  16. Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
    J Leukoc Biol. 85 (1): 44-54.
  17. Miyahara, T. et al. (2013) D-series resolvin attenuates vascular smooth muscle cell activation and neointimal hyperplasia following vascular injury.
    FASEB J. 27 (6): 2220-32.
  18. Vasicek, J. et al. (2015) Determination of Lymphocyte Subset Distribution in the Peripheral Blood of Rabbits Immunized with CFA.
    Int Symp Anim Sci UDC:639.112 pp. 226-31.
  19. Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
    Journal of Central European Agriculture. 18 (1): 226-34.
  20. Vašíček, J et al. (2018) The Efficiency of Immunomagnetic Sorting of Rabbit Bone Marrow Celld for the Establishment of Mesenchymal Stem Cell Culture.
    Journal of Microbiology, Biotechnology and Food Sciences. 8 (3): 890-2.
  21. Vašíček, J et al. (2014) Basic Blood Analysis of Rabbits Immunized with Vaccine Against Myxomatosis.
    Proc Int Symp Anim Sci 2014: 411-6.
  22. Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
    Veterinární Medicína. 61 (No. 10): 571-6.
  23. Barth, H. et al. (2019) Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
    Graefes Arch Clin Exp Ophthalmol. 257 (4): 769-83.
  24. Kovac, M. et al. (2017) Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells.
    Biotechnol Prog. 33 (6): 1601-13.
  25. Desando, G. et al. (2021) Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care.
    Stem Cell Rev Rep. Jan 19 [Epub ahead of print].
  26. Kulikova, B. et al. (2019) Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.
    Acta Histochem. 121 (4): 491-9.
  27. Adly, H. et al. (2021) Repopulation of multipotent stem cells derived from adult male rabbits on a polycaprolactone scaffold: an in vitro. study
    Al-Azhar International Medical Journal. 2 (11): 37-42.

Immunofluorescence

Synonyms
LCA
RRID
AB_10961760
View more products with CD45 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rabbit Products