Protocol: PK ELISA Antigen Capture Format For Use With Anti-Omalizumab Antibody
Pharmacokinetic (PK) ELISA Antigen Capture Format: For use with anti-omalizumab monoclonal antibody product HCA237P or HCA238
This method provides a procedure for carrying out a PK ELISA Antigen Capture Format with anti-omalizumab antibodies, product codes HCA237P or HCA238 (detection antibody), and using omalizumab monoclonal antibody for the standard curve.
Anti-omalizumab drug/target complex antibody recognizes omalizumab only when bound to its target IgE (HCA171). It does not recognize the free drug or unbound IgE. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
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