Protocol: PK Antigen Capture ELISA For Use With Anti-Infliximab Antibodies
Pharmacokinetic (PK) Antigen Capture ELISA: For Use with Anti-Infliximab Monoclonal Antibody catalog TZA009
This method provides a procedure for carrying out a PK ELISA Antigen Capture Format with Anti-Infliximab Antibody, TZA009 (detection antibody), and using infliximab for the standard curve. Anti-Infliximab drug/target complex antibody recognizes infliximab only when bound to its target human TNFα. It does not recognize the free drug or unbound human TNFα. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.