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Protocol: PK Antigen Capture ELISA for Use with Anti-Ranibizumab Antibodies
Pharmacokinetic (PK) Antigen Capture ELISA: for Use with Anti-Ranibizumab Monoclonal Antibodies HCA304, HCA306 and HCA307
This method provides a procedure for carrying out a PK antigen capture ELISA with Anti-Ranibizumab Antibodies, catalog numbers HCA304, HCA306 or HCA307, and using ranibizumab for the standard curve. Anti-Ranibizumab/VEGF Antibody HCA304 recognizes ranibizumab only when bound to its target, human VEGF-A. Anti-Ranibizumab Antibodies HCA306 and HCA307 are non-inhibitory and bind the drug alone and the drug in complex with VEGF-A. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.