Protocol: PK Antigen Capture ELISA for Use with Anti-Ranibizumab Antibody HCA316P
Pharmacokinetic (PK) Antigen Capture ELISA: for use with Anti-Ranibizumab Monoclonal Antibody HCA316P
This method provides a procedure for carrying out a PK antigen capture ELISA with Anti-Ranibizumab Antibody, catalog number HCA316P (HRP conjugated detection antibody), and using ranibizumab for the standard curve. Anti-Ranibizumab Antibody HCA316P is a non-inhibitory, anti-idiotypic antibody that binds to ranibizumab, but does not inhibit the binding of ranibizumab to VEGF-A. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.