Human IgE | AbD264-hIgE

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Human IgE | AbD264-hIgE gallery image 1

Recombinant Human IgE (HCA171) used as an antigen in an indirect ELISA followed by Mouse anti Human IgE (0100-0413) and Rabbit F(ab)'2 anti Mouse IgG:HRP (STAR13B) as detection reagents

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Human IgE | AbD264-hIgE gallery image 2

SDS-PAGE analysis of recombinant Human IgE lambda (HCA171). The sample was analyzed under reducing, denaturing conditions (Lane 2) and non-reducing, denaturing conditions (Lane 3) and visualized using Coomassie staining. The heavy (HC) and light chains (LC) are indicated, respectively

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Human IgE | AbD264-hIgE gallery image 3

Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody (0100-0413) as a capture reagent and biotinylated Goat anti Human IgE antibody (STAR147B) as a detection reagent with recombinant human IgE (HCA171) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma nd serum samples diluted 1:5 are indicated in green and orange respectively

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  • Recombinant Human IgE Lambda
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
  • Clone
    AbD264-hIgE
  • Isotype
    IgE Lambda
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA171Edatasheet pdfdatasheet pdf0.1 mg
    HCA171
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Clone AbD264-hIgE contains a lambda light chain.

      Clone AbD264-hIgE is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Product Form
      Human IgE antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      Purified IgE prepared by affinity chromatography.
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein (GFP).
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human IgE Formats

    Formats Clone Applications Sizes available
    Human IgE : Purified AbD264-hIgE E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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