Protocol: PK Antigen Capture ELISA for Use with Anti-Atezolizumab Antibodies
Pharmacokinetic (PK) Antigen Capture ELISA: For use with Anti-Atezolizumab Monoclonal Antibody catalog TZA013
This method provides a procedure for carrying out a PK ELISA antigen capture format with Anti-Atezolizumab Antibody, TZA013 (detection antibody), and using atezolizumab for the standard curve. Anti-atezolizumab drug/target complex antibody recognizes atezolizumab only when bound to its target human programmed cell death ligand 1 (PD-L1). It does not recognize the free drug or unbound human PD-L1. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.