Pharmacokinetic (PK) Antigen Capture ELISA: for Use with Anti-Ipilimumab Monoclonal Antibody TZA001
Protocol: PK Antigen Capture ELISA for Use with Anti-Ipilimumab Antibody
This method provides a procedure for carrying out a PK ELISA antigen capture format with Anti-Ipilimumab Antibody, TZA001 (detection antibody), and using ipilimumab for the standard curve. Anti-ipilimumab drug/target complex antibody recognizes ipilimumab only when bound to its target, human CTLA-4. It does not recognize the free drug or unbound human CTLA-4. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.