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Protocol: PK ELISA Antigen Capture Format For Use With Anti-Trastuzumab Antibody
Pharmacokinetic (PK) ELISA Antigen Capture Format: For use with anti-trastuzumab monoclonal antibody product HCA263
This method provides a procedure for carrying out a PK ELISA Antigen Capture Format with anti-trastuzumab antibody, product code HCA263 (detection antibody), and using trastuzumab monoclonal antibody for the standard curve. Anti-trastuzumab drug/target complex antibody recognizes trastuzumab only when bound to its target human ErbB2. It does not recognize the free drug or unbound human ErbB2. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
Lynx Rapid Conjugation Kit® is a registered trademark of Bio-Rad AbD Serotec Ltd.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.