Mouse IgG2b Negative Control antibody
Mouse IgG2b Negative Control: Amethyst Orange
- Product Type
- Negative/Isotype Control
- Isotype
- IgG2b
- Specificity
- Mouse IgG2b Negative Control
Mouse IgG2b Negative Control is negative on all human cells and cell lines tested. This antibody recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. Test results show that MCA691C is also suitable for use as a negative control with bovine, ovine, porcine, canine and guinea-pig tissues. |
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Amethyst Orange - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Amethyst Orange 405 540 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | * |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
* This antibody should be used at the same concentration as the test antibody.
References for Mouse IgG2b Negative Control antibody
-
Shoham, T. et al. (2001) Reduced expression of activin A in focal lymphoid agglomerates within nasal polyps.
J Histochem Cytochem. 49 (10): 1245-52. -
Zheng, X. et al. (2002) Interleukin-3, but not granulocyte-macrophage colony-stimulating factor and interleukin-5, inhibits apoptosis of human basophils through phosphatidylinositol 3-kinase: requirement of NF-kappaB-dependent and -independent pathways.
Immunology. 107 (3): 306-15. -
Grant, A.J. et al. (2002) Hepatic expression of secondary lymphoid chemokine (CCL21) promotes the development of portal-associated lymphoid tissue in chronic inflammatory liver disease.
Am J Pathol. 160 (4): 1445-55. -
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9. -
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94. -
Lawrence, J. et al. (2024) Porcine Monocyte DNA Traps Formed during Infection with Pathogenic Clostridioides difficile Strains
Pathogens. 13 (3): 228.
MCA691AMO
148938If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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