IgG1 antibody
Phycoerythrin conjugated Goat anti Mouse IgG1 antibody (STAR132PE) used for the detection of bound antibody on lymphoblastoid cell lines by flow cytometry.
Image caption:
Estimation of ΔBNLF2a and wild-type LCLs expressing lytic antigens; recognition by immediate early antigen-specific CD8+ T cells. The proportion of LCLs spontaneously reactivating into lytic cycle was assessed by intracellular BZLF1 staining and analysis by flow cytometry, with representative examples shown for LCLs derived from two different donors: (A) donor 1 and (B) donor 2. Immediate early lytic cycle CD8+ T cell recognition of wild-type (wt), ΔBNLF2a (Δ2a) and ΔBZLF1 (ΔBZ) LCLs using HLA-B*0801-restricted RAK (BZLF1) clones against appropriately HLA matched donor 1 and 2 LCLs (C and D respectively) was measured by IFNγ ELISA. Results using wild-type or ΔBNLF2a cells diluted with ΔBZLF1 cells as appropriate are shown, where arrows indicate equivalent numbers of lytic antigen expressing cells. Experiments were also conducted using HLA-C*0202-restricted IACP (BRLF1) clones against donor 3 LCLs (E), and HLA-B*4501-restricted AEN (BRLF1) clones against donor 4 LCLs (F). For donor 4, both the wild-type and ΔBNLF2a LCLs were diluted with ΔBZLF1 LCL (wild-type-LCL titration data not shown). Data are represented as mean+/−SEM.
From: Croft NP, Shannon-Lowe C, Bell AI, Horst D, Kremmer E, et al.. (2009)
Stage-Specific Inhibition of MHC Class I Presentation by the Epstein-Barr Virus BNLF2a Protein during Virus Lytic Cycle.
PLoS Pathog 5(6): e1000490.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) used for the evaluation of rotavirus specific IgG1 antibodies in mouse serum be ELISA.
Image caption:
Rotavirus-specific IgG-subclass antibodies in serum. Geometric mean titers (GMT) in arbitrary units (AU) ± SEM of the rotavirus-specific IgG-subclass antibodies IgG1, IgG2a, IgG2b and IgG3 in serum individually collected at day 28, depicted per antibody.
From: Knipping et a.l.
A gastrointestinal rotavirus infection mouse model for immune modulation studies
Virology Journal 2011 8:109.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Goat anti Mouse IgG1 antibody (STAR132F) used for the detection of bound Mouse anti BrdU antibody by immunofluorescence.
Image caption:
Sequential labelling using EdU and BrdU. Microscopy pictures of cells double-labelled with EdU and BrdU. Cells synchronized in G1 phase were released and pulse-labelled in two consecutive S-phases, first with EdU, then with BrdU, as described in the text. Samples were harvested after the next mitosis, fixed and processed as described and imaged using fluorescence microscopy.
From: Anda S, Boye E, Grallert B (2014)
Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast. PLoS ONE 9(2): e88629.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Goat anti Mouse IgG1 antibody (STAR132F) used for the detection of bound Mouse anti BrdU antibody by immunofluorescence.
Image caption:
Microscopy pictures of cells labelled with EdU or BrdU. Cells were labelled with either analogue and detection for both analogues was performed to check cross-reactivity.
From: Anda S, Boye E, Grallert B (2014)
Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast.
PLoS ONE 9(2): e88629.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) used as a secondary detection reagent for the evaluation of sheep plasma IgG levels by ELISA.
Image caption:
Distribution of anti-Teladorsagia circumcincta IgG levels with age and sex in Soay sheep.
From: Sparks AM, Watt K, Sinclair R, Pilkington JG, Pemberton JM, McNeilly TN, et al. (2019)
The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries).
PLoS Genet 15(11): e1008461.
doi: 10.1371/journal.pgen.1008461
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Goat anti Mouse IgG1 antibody (STAR132F) as a secondary antibody to detect Mouse anti IdU in an immunofluorescent assay.
Image caption:
Granddaughter cells inherit a mixture of old and new DNA. (a) Quantification of CldU (1st S phase) and IdU (2nd S phase) incorporation in 10 cell pairs. (b) An example of four-celled mycelium after CldU and IdU labelling. Note the uneven distribution of CldU and even distribution of IdU.
From:Anda S, Boye E, Schink KO, Grallert B.
Cosegregation of asymmetric features during cell division.
Open Biol. 2021 Aug;11(8):210116.
doi: 10.1098/rsob.210116.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Mouse IgG1:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG1
Antibody Quality and Performance Guarantee
| Goat anti Mouse IgG1 antibody recognizes Mouse IgG1. This antibody has been cross absorbed against mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG1 antibody shows minimal cross-reactivity with human immunoglobulins. Goat anti Mouse IgG1 antibody has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology. |
|
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Antiserum Preparation
- Antisera to mouse IgG1 were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Mouse IgG1 paraproteins.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG1 antibody
-
Croft, N.P. et al. (2009) Stage-specific inhibition of MHC class I presentation by the Epstein-Barr virus BNLF2a protein during virus lytic cycle.
PLoS Pathog. 5(6): e1000490. -
Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting MHC class I molecules trafficking on both the exocytic and endocytic pathways.
J Virol. 85: 1604-14. -
Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
Virol J. 8: 109. -
Young, D. et al. (2012) Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis.
J Nutr. 142 (2): 363-8. -
Bagai, U. and Pawar, A. (2013) A blood stage fraction of Plasmodium berghei induces protective and long lasting immune response in BALB/c mice.
Parasitol Int. 62: 329-36. -
Anda, S. et al. (2014) Cell-cycle analyses using thymidine analogues in fission yeast.
PLoS One. 9 (2): e88629. -
Kamat, M.M. et al. (2016) Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy.
Biol Reprod. 95 (3): 68. -
Ramanathan, R. et al. (2015) Transplantation of human stem cell-derived hepatocytes in an animal model of acute liver failure.
Surgery. 158 (2): 349-59.
View The Latest Product References
-
Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
J Toxicol Environ Health A. 78 (8): 516-23. -
Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
Sci Rep. 5: 12371. -
Gutiérrez-Miranda, B. et al. (2020) Oleacein Attenuates the Pathogenesis of Experimental Autoimmune Encephalomyelitis through Both Antioxidant and Anti-Inflammatory Effects.
Antioxidants (Basel). 9 (11): 1161. -
Gutierrez, B. et al. (2020) Oleanolic acid ameliorates intestinal alterations associated with EAE.
J Neuroinflammation. 17 (1): 363. -
Apóstolo, N. et al. (2020) Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer.
Nat Commun. 11 (1): 5171. -
Zhuang, X. et al. (2020) CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth.
JCI Insight. 5 (19): e138808. -
Sparks, A.M. et al. (2018) Natural Selection on Antihelminth Antibodies in a Wild Mammal Population.
Am Nat. 192 (6): 745-760. -
Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
Parasitol Res. 118 (6): 1849-63. -
Kim, Y.H. et al. (2019) Effects of ruminal pH on gene expression in the rumen epithelium, peripheral blood mononuclear cell subpopulations, and blood metabolites from Holstein calves during weaning transition.
J Vet Med Sci. 81 (6): 808-816. -
Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
Vet Immunol Immunopathol. 211: 44-48. -
Sparks, A.M. et al. (2019) The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries.).
PLoS Genet. 15 (11): e1008461. -
Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
Front Immunol. 10: 579. -
Verma, R. et al. (2018) Leishmania donovani. molecules recognized by sera of filaria infected host facilitate filarial infection.
Parasitol Res. 117 (9): 2901-12. -
Gatkowska, J. et al. (2019) The Impact of the Antigenic Composition of Chimeric Proteins on Their Immunoprotective Activity against Chronic Toxoplasmosis in Mice.
Vaccines (Basel). 7(4):154. -
Han, H.J. et al. (2022) Metal arsenic mediated enhancement of type-2 immunity in brains with altered locomotive activities in mice with autism-like behavioral characteristics.
Toxicol Res. 38 (1): 27-33. -
Anda, S. et al. (2021) Cosegregation of asymmetric features during cell division.
Open Biol. 11 (8): 210116. -
Minaei, S. et al. (2018) Propranolol efficacy as a novel adjuvant for immunization against Toxoplasma gondii. tachyzoites.
Exp Parasitol. 194: 60-66. -
Ngoepe, T.A. et al. (2023) Th1 and Th2 epitopes of Cowdria polymorphic gene 1 of Ehrlichia ruminantium.
Onderstepoort J Vet Res. 90 (1): e1-e15. -
Jo, J. et al. (2023) Immunodysregulatory potentials of polyethylene or polytetrafluorethylene microplastics to mice subacutely exposed via intragastric intubation.
Toxicol Res. 39 (3): 419-27. -
Sessevmez, M. et al. (2023) Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
J Drug Deliv Sci Technol. 86: 104704. -
Veisi, R. et al. (2024) Expression and purification of TolC as a recombinant protein vaccine against Shigella flexneri and evaluation of immunogenic response in mice.
Microb Pathog. : 106539 [Epub ahead of print] -
Faber, E. et al. (2024) Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3.
Vaccine. 42 (2): 136-145. -
Pretorius, A. et al. (2024) Screening for immune biomarkers associated with infection or protection against Ehrlichia ruminantium by RNA-sequencing analysis.
Microb Pathog. : 106588. -
Kusma, S. et al. (2024) Oral subacute polypropylene microplastics administration effect on potential immunotoxicity in ICR mice.
J Toxicol Environ Health A. : 1-10 [Epub ahead of print]. -
Wu, Y. et al. (2020) Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccine
bioRχiv . [Epub ahead of print].
- RRID
- AB_1102649
- UniProt
- P01869
- P01868
- Entrez Gene
- Ighg1
- Ighg1
- GO Terms
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0003823 antigen binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- View More GO Terms
- GO:0050766 positive regulation of phagocytosis
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
STAR132PE
151750
162641
168631
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