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CD40 antibody | IL-A156

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Anti Bovine CD40 Antibody, clone IL-A156 thumbnail image 1 Anti Bovine CD40 Antibody, clone IL-A156 thumbnail image 2 Anti Bovine CD40 Antibody, clone IL-A156 thumbnail image 3 Anti Bovine CD40 Antibody, clone IL-A156 thumbnail image 4 Anti Bovine CD40 Antibody, clone IL-A156 thumbnail image 5
Anti Bovine CD40 Antibody, clone IL-A156 gallery image 1
Figure A. RPE conjugated mouse anti bovine CD32 (MCA5918PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD32 (MCA5918PE) and FITC conjugated mouse anti bovine CD40 (MCA2431F). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD40 Antibody, clone IL-A156 gallery image 2
Published customer image:
Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431GA) used for the identification of CD40 expressing lymph node cells from naïve and contagious bovine pleuropneumonia infected cattle
Image caption:
Effect of free Mmm galactan on surface expression of CD40 ( A ) and CD80 ( B ) on bovine macrophages. Cells were incubated for 48 h with galactan (EPS) at 0.1, 1 and 10 μg/ml, or with LPS and medium only (C−) as positive and negative controls respectively. Cell-surface expression of CD40/CD80 was measured by flow cytometry and results expressed as mean (±SD) percentages of positive cells for a given marker (n = 3). Asterisks indicate statistically significant differences (p < 0.05) with the negative control.

From: Totté P, Puech C, Rodrigues V, Bertin C, Manso-Silvan L, Thiaucourt F.
Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
Vet Res. 2015 Oct 21;46(1):122.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine CD40 Antibody, clone IL-A156 gallery image 3
Figure A. Purified mouse anti bovine CD1w3 (MCA1647G) detected with goat anti mouse IgG2b RPE (STAR134PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Purified mouse anti bovine CD1w3 (MCA1647G) detected with goat anti mouse IgG2b RPE (STAR134PE) and FITC conjugated mouse anti bovine CD40 (MCA2431F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD40 Antibody, clone IL-A156 gallery image 4
Figure A. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified mouse IgG2b isotype control (MCA691) detected with RPE conjugated goat anti mouse IgG2b (STAR134PE). Figure B. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified CD1w3 (MCA1647G) detected with RPE conjugated goat anti mouse IgG2b (MCASTAR134PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD40 Antibody, clone IL-A156 gallery image 5
Published customer image:
Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431GA) used to assess CD40 expression on bovine PBMCs by flow cytometry.
Image caption:
Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and ​and2C.2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14−CD16++ (red), CD14+CD16+ (blue) and CD14+CD16low/- (orange) compared to PBMC (black). Data shown are for one representative animal of four animals.

From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y, Entrican G, Glass EJ.
Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 2015 Sep 25;46:112.
doi: 10.1186/s13567-015-0246-4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Bovine CD40:FITC

Product Type
Monoclonal Antibody
Clone
IL-A156
Isotype
IgG1
Specificity
CD40

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Product Code Applications Pack Size List Price Your Price Qty
MCA2431F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD40 antibody, clone IL-A156 recognizes the bovine Tumor necrosis factor receptor superfamily member 5, also known as CD40 or B-cell surface antigen CD40. CD40 is a 260 amino acid single pass type 1 transmembrane glycoprotein with a predicted molecular mass of 31 kDa. Bovine CD40 also has an N terminal signal peptide of 20 amino acids. Bovine CD40 is expressed by B lymphocytes and a subset of T lymphocytes.

CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD40 antibody

  1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
    Scand J Immunol. 61 (3): 242-50.
  2. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
    J Leukoc Biol. 76 (3): 553-61.
  3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84 (Pt 7): 1771-80.
  4. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
    Immunology 108: 55- 61.
  5. Haas, K.M. et al. (2001) Enhancing effects of anti-CD40 treatment on the immune response of SCID-bovine mice to Trypanosoma congolense infection.
    J Leukoc Biol. 70: 931-40.
  6. Totté, P. et al. (2015) Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
    Vet Res. 46 (1): 122.
  7. Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
    Vet Res. 46: 112.
  8. Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
    Vet Immunol Immunopathol. 19 Nov: 110363.
    9. View The Latest Product References
  9. Kornuta, C.A. et al. (2021) MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1.
    Transbound Emerg Dis. 68 (2): 587-97.
  10. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.

Flow Cytometry

UniProt
Q28203
Entrez Gene
CD40
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA2431F

148236

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