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CD8 Beta antibody | CC58

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Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 1 Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 2 Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 3 Anti Bovine CD8 Beta Antibody, clone CC58 thumbnail image 4
Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 1
Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD21 (MCA1424F) and RPE conjugated Mouse anti Bovine CD8b (MCA1654PE). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 2
Figure A. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti bovine CD8b (MCA1645PE) and FITC conjugated mouse anti bovine CD21 (MCA5953F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 3
Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA5953F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD21 (MCA5953F) and RPE conjugated mouse anti bovine CD8b (MCA1654PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD8 Beta Antibody, clone CC58 gallery image 4
Figure A. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and Mouse anti Bovine CD8 Beta (MCA1654GA) detected with Goat anti Mouse IgG1:RPE (STAR132PE). All experiments performed on bovine red blood cell lysed blood gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Bovine CD8 Beta:RPE

Product Type
Monoclonal Antibody
Clone
CC58
Isotype
IgG1
Specificity
CD8 Beta

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Product Code Applications Pack Size List Price Your Price Qty
MCA1654PE
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SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Mouse anti Bovine CD8 beta antibody, clone CC58 recognizes an epitope associated with the bovine CD8 beta chain.

CD8 is usually expressed as an α/β heterodimer. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules.

Mouse anti Bovine CD8 beta antibody, clone CC58 has been successfully used for the immunohistochemical detection of CD8 on formalin fixed, paraffin embedded placental tissue from water buffalo (Cantón et al. 2014).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Bovine leucocytes
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD8 Beta antibody

  1. Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.
    Microbes Infect. 10: 1370-8.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
    Immunol Today. 18 (8): 365-8.
  4. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
    Vet Immunol Immunopathol. 27 (1-3): 28-30.
  5. Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.
    J Virol. 83: 4039-50.
  6. Gerner, W. et al. (2010) Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.
    Vet Immunol Immunopathol. 138: 154-8.
  7. MacHugh, N.D. and Sopp, P. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).
    Vet Immunol Immunopathol. 27: 65-9.
  8. Soltys, J. and Quinn, M.T. (1999) Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression.
    Infect Immun. 67: 6293-302.
    9. View The Latest Product References
  9. Cantón, G.J. et al. (2014) Characterization of immune cell infiltration in the placentome of water buffaloes (Bubalus bubalis) infected with neospora caninum during pregnancy.
    J Comp Pathol. 150: 463-8.
  10. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
  11. Hecker, Y.P. et al. (2015) Cell mediated immune responses in the placenta following challenge of vaccinated pregnant heifers with Neospora caninum.
    Vet Parasitol. 214 (3-4): 247-54.
  12. Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis. in Canchim calves.
    Vet Immunol Immunopathol. 230: 110132.

Flow Cytometry

RRID
AB_322962
UniProt
A7YW30

MCA1654PE

147814

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