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IgG2b antibody

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Anti Mouse IgG2b Antibody thumbnail image 1 Anti Mouse IgG2b Antibody thumbnail image 2
Anti Mouse IgG2b Antibody gallery image 1
Figure A. Purified mouse anti bovine CD1w3 (MCA1647G) detected with goat anti mouse IgG2b RPE (STAR134PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Purified mouse anti bovine CD1w3 (MCA1647G) detected with goat anti mouse IgG2b RPE (STAR134PE) and FITC conjugated mouse anti bovine CD40 (MCA2431F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Mouse IgG2b Antibody gallery image 2
Figure A. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified mouse IgG2b isotype control (MCA691) detected with RPE conjugated goat anti mouse IgG2b (STAR134PE). Figure B. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified CD1w3 (MCA1647G) detected with RPE conjugated goat anti mouse IgG2b (MCASTAR134PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Goat anti Mouse IgG2b:Alk. Phos.

Goat anti Mouse IgG2b:FITC

Goat anti Mouse IgG2b:HRP

Goat anti Mouse IgG2b

Goat anti Mouse IgG2b:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR134A
Datasheet
C E P WB
SDS
1 ml loader

STAR134F
Datasheet
C F IF
SDS
0.5 mg loader

STAR134P
Datasheet
C E P WB
SDS
1 ml loader

STAR134
Datasheet
E
SDS
0.5 mg

STAR134PE
Datasheet
F
SDS
0.1 mg loader

Goat anti Mouse IgG2b antibody recognizes Mouse IgG2b as has been cross absorbed against mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2b antibody shows minimal cross-reactivity with human immunoglobulins.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to alkaline phosphatase - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Antiserum Preparation
Antisera to mouse IgG2b were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
50mM TRIS
1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
50% Glycerol
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
50% Glycerol
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Immunogen
Mouse IgG2b paraproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
12 months from date of despatch
Guarantee
6 months from date of despatch

More Information

UniProt
P01867
Entrez Gene
Ighg2b
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
Regulatory
For research purposes only

Applications of IgG2b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Flow Cytometry 1/1000
Immunofluorescence Neat 1/10
Immunohistology - Frozen
ELISA 1/4000 1/8000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
ELISA
Flow Cytometry 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgG2b antibody

  1. Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
    Virol J. 8: 109.
  2. Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
    J Toxicol Environ Health A. 78 (8): 516-23.
  3. Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
    Sci Rep. 5: 12371.
  4. Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
    Parasitol Res. 118 (6): 1849-63.

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