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IgG2b antibody

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Anti Mouse IgG2b Antibody thumbnail image 1 Anti Mouse IgG2b Antibody thumbnail image 2
Anti Mouse IgG2b Antibody gallery image 1
Figure A. Purified mouse anti bovine CD1w3 (MCA1647G) detected with goat anti mouse IgG2b RPE (STAR134PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Purified mouse anti bovine CD1w3 (MCA1647G) detected with goat anti mouse IgG2b RPE (STAR134PE) and FITC conjugated mouse anti bovine CD40 (MCA2431F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Mouse IgG2b Antibody gallery image 2
Figure A. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified mouse IgG2b isotype control (MCA691) detected with RPE conjugated goat anti mouse IgG2b (STAR134PE). Figure B. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified CD1w3 (MCA1647G) detected with RPE conjugated goat anti mouse IgG2b (MCASTAR134PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Goat anti Mouse IgG2b:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG2b

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Product Code Applications Pack Size List Price Your Price Qty
STAR134PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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Goat anti Mouse IgG2b antibody recognizes Mouse IgG2b as has been cross absorbed against mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2b antibody shows minimal cross-reactivity with human immunoglobulins.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Antiserum Preparation
Antisera to mouse IgG2b were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Immunogen
Mouse IgG2b paraproteins.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

References for IgG2b antibody

  1. Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
    Virol J. 8: 109.
  2. Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
    J Toxicol Environ Health A. 78 (8): 516-23.
  3. Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
    Sci Rep. 5: 12371.
  4. Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
    Parasitol Res. 118 (6): 1849-63.

RRID
AB_1102660
UniProt
P01867
Entrez Gene
Ighg2b
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region

STAR134PE

155483 159009

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