IgG2b antibody
Horseradish Goat anti Mouse IgG2b antibody (STAR!34P) used to detect bound Mouse anti Nrf2 antibodies in a western blotting assay.
Image caption:
Partial knock-down of p62 does not change human synovial fibroblast fate upon BAY 11-7085 treatment
(C) Synovial fibroblasts were cultured with different concentrations of MG132, in the presence or absence of serum, for 24 hours. Western blots show expression of P-S349 p62, p62, Nrf2, Nrf2 phosphorylated on Serine 40 and GAPDH in cell extracts of one representative OA patient. Experiments were repeated three times with cells from three different OA patients.
From:Relic B, Charlier E, Deroyer C, Malaise O, Crine Y, Neuville S, Gillet P, de Seny D, Malaise MG.
Serum starvation raises turnover of phosphorylated p62/SQSTM1 (Serine 349), reveals expression of proteasome and N-glycanase1 interactive protein RAD23B and sensitizes human synovial fibroblasts to BAY 11-7085-induced cell death.
Oncotarget. 2018 Nov 9;9(88):35830-35843.
doi: 10.18632/oncotarget.26295.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Mouse IgG2b:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG2b
Antibody Quality and Performance Guarantee
| Goat anti Mouse IgG2b antibody recognizes Mouse IgG2b as has been cross absorbed against mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2b antibody shows minimal cross-reactivity with human immunoglobulins. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Antiserum Preparation
- Antisera to mouse IgG2b were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Mouse IgG2b paraproteins.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG2b antibody
-
Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
Virol J. 8: 109. -
Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
J Toxicol Environ Health A. 78 (8): 516-23. -
Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
Sci Rep. 5: 12371. -
Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
Parasitol Res. 118 (6): 1849-63. -
Shi, F. et al. (2020) The changes of immune-related molecules within the ileal mucosa of piglets infected with porcine circovirus type 2.
J Vet Sci. 21 (5): e78. -
Relic, B. et al. (2018) Serum starvation raises turnover of phosphorylated p62/SQSTM1 (Serine 349), reveals expression of proteasome and N-glycanase1 interactive protein RAD23B and sensitizes human synovial fibroblasts to BAY 11-7085-induced cell death.
Oncotarget. 9 (88): 35830-43.
- RRID
- AB_1102660
- UniProt
- P01867
- Entrez Gene
- Ighg2b
- GO Terms
- GO:0005886 plasma membrane
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
STAR134PE
155483
159009
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