CD19 antibody | Bu12

Mouse anti Human CD19:FITC

Product Type
Monoclonal Antibody
Clone
Bu12
Isotype
IgG1
Specificity
CD19

Product Code Applications Pack Size List Price Your Price Qty
MCA2495F
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SDS Safety Datasheet SDS
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Mouse anti Human CD19 antibody, clone Bu12 recognizes human CD19, a 95 kDa cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Human EB-4 Burkitt lymphoma cell line
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells from the X63 AG8 653 plasmacytoma
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD19 antibody

  1. Zhou, L. and Tedder, T. (1993) CD19 Workshop panel report.
    In leucocyte Typing V. White cell differentiation antigens. Oxford University press. p507 – 509.
  2. Smith SH et al. (1991) Activation of human B cells through the CD19 surface antigen results in homotypic adhesion by LFA-1-dependent and -independent mechanisms.
    Immunology. 73 (3): 293-7.
  3. Callard, R.E. et al. (1992) CD19 regulation of human B cell responses. B cell proliferation and antibody secretion are inhibited or enhanced by ligation of the CD19 surface glycoprotein depending on the stimulating signal used.
    J Immunol. 148 (10): 2983-7.
  4. Callard, R.E. et al. (1995) CD40 cross-linking inhibits specific antibody production by human B cells.
    Int Immunol. 7 (11): 1809-15.
  5. Flavell, D.J. et al. (1995) Preclinical studies with the anti-CD19-saporin immunotoxin BU12-SAPORIN for the treatment of human-B-cell tumours.
    Br J Cancer. 72 (6): 1373-9.
  6. Vallera, D. A. et al. (2004) Radiotherapy of CD19 expressing Daudi tumors in nude mice with Yttrium-90-labeled anti-CD19 antibody.
    Cancer Biother Radiopharm. 19 (1): 11-23.

RRID
AB_844481
UniProt
P15391
Entrez Gene
CD19
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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