CD19 antibody | Bu12
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|Mouse anti Human CD19 antibody, clone Bu12 recognizes human CD19, a 95 kDa cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.|
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
- Human EB-4 Burkitt lymphoma cell line
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells from the X63 AG8 653 plasmacytoma
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD19 antibody
Zhou, L. and Tedder, T. (1993) CD19 Workshop panel report.
In leucocyte Typing V. White cell differentiation antigens. Oxford University press. p507 – 509.
Smith SH et al. (1991) Activation of human B cells through the CD19 surface antigen results in homotypic adhesion by LFA-1-dependent and -independent mechanisms.
Immunology. 73 (3): 293-7.
Callard, R.E. et al. (1992) CD19 regulation of human B cell responses. B cell proliferation and antibody secretion are inhibited or enhanced by ligation of the CD19 surface glycoprotein depending on the stimulating signal used.
J Immunol. 148 (10): 2983-7.
Callard, R.E. et al. (1995) CD40 cross-linking inhibits specific antibody production by human B cells.
Int Immunol. 7 (11): 1809-15.
Flavell, D.J. et al. (1995) Preclinical studies with the anti-CD19-saporin immunotoxin BU12-SAPORIN for the treatment of human-B-cell tumours.
Br J Cancer. 72 (6): 1373-9.
Vallera, D. A. et al. (2004) Radiotherapy of CD19 expressing Daudi tumors in nude mice with Yttrium-90-labeled anti-CD19 antibody.
Cancer Biother Radiopharm. 19 (1): 11-23.
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