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CD14 antibody | UCHM1

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Anti Human CD14 Antibody, clone UCHM1 thumbnail image 1 Anti Human CD14 Antibody, clone UCHM1 thumbnail image 2 Anti Human CD14 Antibody, clone UCHM1 thumbnail image 3 Anti Human CD14 Antibody, clone UCHM1 thumbnail image 4 Anti Human CD14 Antibody, clone UCHM1 thumbnail image 5
Anti Human CD14 Antibody, clone UCHM1 gallery image 1
Figure A. FITC conjugated mouse anti human CD9 (MCA469F) and Alexa Fluor700 conjugated mouse IgG2a isotype control (MCA929A700). Figure B. FITC conjugated mouse anti human CD9 (MCA469F) and Alexa Fluor700 conjugated mouse anti human CD14 (MCA596A700). All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD14 Antibody, clone UCHM1 gallery image 2
Figure A. FITC conjugated mouse anti human CD66b (MCA216F) and APC conjugated mouse IgG1 isotype control (MCA928APC). Figure B. FITC conjugated mouse anti human CD66b (MCA216F) and APC conjugated mouse anti human CD14 (MCA596APC). All experiments performed on red cell lysed human peripheral blood gated on myeloid cells in the presence of Human SeroBlock (BUF070A).
Anti Human CD14 Antibody, clone UCHM1 gallery image 3
Figure A. RPE conjugated mouse anti human CD9 (MCA469PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated mouse anti human CD9 (MCA469PE) and FITC conjugated mouse anti human CD14 (MCA596F). All experiments performed on human Peripheral blood mononuclear cells in the presence of Human SeroBlock (BUF070A).
Anti Human CD14 Antibody, clone UCHM1 gallery image 4
Figure A. Pacific Blue conjugated mouse anti human CD9 (MCA469PB) and Alexa Fluor488 conjugated mouse IgG2a isotype control (MCA929A488). Figure B. Pacific Blue conjugated mouse anti human CD9 (MCA469PB) and Alexa Fluor488 conjugated mouse anti human CD14 (MCA596A488). All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD14 Antibody, clone UCHM1 gallery image 5
Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (MCA2185) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1 (MCA596B) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively.

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Mouse anti Human CD14:FITC

Product Type
Monoclonal Antibody
Clone
UCHM1
Isotype
IgG2a
Specificity
CD14

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MCA596F
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Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative  (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Fish
Trout
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human Thymocytes followed by peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD14 antibody

  1. Hogg, N. & Horton, M.A. (1987) Myeloid antigens: new and previously defined clusters
    in Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J.,et al.
  2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  3. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.
    Leucocyte Typing IV. Oxford University Press p 1058-63.
  4. Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.
    Immunology. 53 (4): 753-67.
  5. Linch, D.C. et al. (1984) Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.
    Blood. 63 (3): 566-73.
  6. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
    J Immunol. 176 (10): 5730-4.
  7. Köller, M. et al. (2004) Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients.
    Int Immunol. 16: 1595-604.
  8. Kämmerer, U. et al. (2003) Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy.
    Am J Pathol. 162: 887-96.
    9. View The Latest Product References
  9. Goddard, S. et al. (2004) Interleukin-10 secretion differentiates dendritic cells from human liver and skin.
    Am J Pathol. 164: 511-9.
  10. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.
    Arterioscler Thromb Vasc Biol. 28: 1491-8.
  11. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12:2.
  12. Hsu, T.L. et al. (2002) Modulation of dendritic cell differentiation and maturation by decoy receptor 3.
    J Immunol. 168: 4846-53.
  13. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.
    Infect Immun. 70: 6688-96.
  14. Din JN et al. (2013) Effect of ω-3 fatty acid supplementation on endothelial function, endogenous fibrinolysis and platelet activation in male cigarette smokers.
    Heart. 99 (3): 168-74.
  15. Fischer, U. and Koellner, B. (2007) Cross-reactivity of human leukocyte differentiation antigen monoclonal antibodies on carp and rainbow trout cells.
    Vet Immunol Immunopathol. 119: 142-55.
  16. Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
    Blood. 106: 3567-74.
  17. Angel, C.E. et al. (2009) Distinctive localization of antigen-presenting cells in human lymph nodes.
    Blood. 113: 1257-67.
  18. Chang, Y.C. et al. (2004) Modulation of macrophage differentiation and activation by decoy receptor 3.
    J Leukoc Biol. 75: 486-94.
  19. Iking-Konert, C. et al. (2008) T lymphocytes in patients with primary vasculitis: expansion of CD8+ T cells with the propensity to activate polymorphonuclear neutrophils.
    Rheumatology (Oxford). 47: 609-16.
  20. Brook, F.A. et al. (2010) Derivation and characterisation of the human embryonic stem cell line, OxF1.
    In Vitro Cell Dev Biol Anim. 46: 173-7.
  21. Bromberek, J.L. et al. (2016) Breed Distribution and Clinical Characteristics of B Cell Chronic Lymphocytic Leukemia in Dogs.
    J Vet Intern Med. 30 (1): 215-22.
  22. Amouroux, R. et al. (2016) De novo DNA methylation drives 5hmC accumulation in mouse zygotes.
    Nat Cell Biol. 18 (2): 225-33.
  23. Spiller, K.L. et al. (2016) Differential gene expression in human, murine, and cell line-derived macrophages upon polarization.
    Exp Cell Res. 347 (1): 1-13.
  24. Kannegieter, N.M. et al. (2018) Analysis of NFATc1 amplification in T cells for pharmacodynamic monitoring of tacrolimus in kidney transplant recipients.
    PLoS One. 13 (7): e0201113.
  25. Matsusaka, K. et al. (2022) Distinct roles in phagocytosis of the early and late increases of cell surface calreticulin induced by oxaliplatin
    Biochem Biophys Rep. 29: 101222.
  26. Wu, T.C. et al. (2018) IL1 Receptor Antagonist Controls Transcriptional Signature of Inflammation in Patients with Metastatic Breast Cancer.
    Cancer Res. 78 (18): 5243-58.
  27. Hoang, P.T. et al. (2018) Subtype Diversification and Synaptic Specificity of Stem Cell-Derived Spinal Interneurons.
    Neuron. 100 (1): 135-149.e7.

Flow Cytometry

Functional Assays

Immunohistology - Frozen

RRID
AB_321314
UniProt
P08571
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0001530 lipopolysaccharide binding
GO:0001847 opsonin receptor activity
GO:0006915 apoptosis
GO:0006909 phagocytosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0031225 anchored to membrane
GO:0016019 peptidoglycan receptor activity
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0070891 lipoteichoic acid binding
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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