CD21 antibody | CC51





Filter by Application:
F ResetMouse anti Bovine CD21:FITC
- Product Type
- Monoclonal Antibody
- Clone
- CC51
- Isotype
- IgG2b
- Specificity
- CD21
Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012). Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig African Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Bovine (Friesian cattle) mesenteric lymph node cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests |
|
Log in | ||
List Price | Your Price | ||||||
|
Log in | ||||||
Description | Mouse IgG2b Negative Control:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Pig wCD8 Alpha:RPE | ||||||
Rat anti Human CD3:Pacific Blue® | MCA1477PB | F * | 100 Tests/1ml |
|
Log in | ||
List Price | Your Price | ||||||
|
Log in | ||||||
Description | Rat anti Human CD3:Pacific Blue® | ||||||
Mouse anti Human CD14:Pacific Blue® | MCA1568PB | F | 100 Tests/1ml |
|
Log in | ||
List Price | Your Price | ||||||
|
Log in | ||||||
Description | Mouse anti Human CD14:Pacific Blue® | ||||||
Mouse anti Human CD14:RPE | MCA1568PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Human CD14:RPE | ||||||
Mouse anti Bovine CD4:RPE | MCA1653PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Bovine CD4:RPE | ||||||
Mouse anti Pig CD25 | MCA1736GA | C F | 0.1 mg |
|
Log in | ||
List Price | Your Price | ||||||
|
Log in | ||||||
Description | Mouse anti Pig CD25 | ||||||
Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Pig CD4 Alpha:RPE | ||||||
Mouse anti Pig SLA Class II DR | MCA2314GA | C F IP | 0.1 mg |
|
Log in | ||
List Price | Your Price | ||||||
|
Log in | ||||||
Description | Mouse anti Pig SLA Class II DR | ||||||
Mouse anti Bovine CD25:RPE | MCA2430PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Bovine CD25:RPE | ||||||
Mouse anti Bovine MHC Class II DR:RPE | MCA5656PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Bovine MHC Class II DR:RPE | ||||||
Mouse anti Pig CD3 | MCA5951GA | C F IP WB | 0.1 mg |
|
Log in | ||
List Price | Your Price | ||||||
|
Log in | ||||||
Description | Mouse anti Pig CD3 | ||||||
Mouse anti Pig CD3:RPE | MCA5951PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Pig CD3:RPE |
Source Reference
-
Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes.
Immunology. 69 (4): 525-30.
References for CD21 antibody
-
Denham S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.
Vet Immunol Immunopathol. 43: 259-67. -
Boersma W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80: 63-78. -
Tenaya I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
Vet Immunol Immunopathol. 149: 167-76. -
Sinkora, M et al. (2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.
Dev Comp Immunol. 39: 409-18. -
Sinkora, M. et al. (2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.
Vet Res. 45: 91. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Li, J. et al. (2023) Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells.
Genes Genomics. Nov 18 [Epub ahead of print]. -
Seemann, L. et al. (2024) Dietary L-carnitine supplementation modifies blood parameters of mid-lactating dairy cows during standardized lipopolysaccharide-induced inflammation.
Front Immunol. 15: 1390137.
View The Latest Product References
-
Yuan, C. et al. (2024) Effects of porcine epidemic diarrhea virus infection on CD21(+) B cells activation.
Vet Microbiol. 293: 110087.
- Synonyms
- CR2
- UniProt
- Q8HY44
MCA5953F


If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Bovine ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up