Mouse IgG2b Negative Control antibody

Mouse IgG2b Negative Control

Product Type
Negative/Isotype Control
Isotype
IgG2b
Specificity
Mouse IgG2b Negative Control

Product Code Applications Pack Size List Price Your Price Qty
MCA691
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Mouse IgG2b Negative Control is negative on all human cells and cell lines tested. This antibody recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Test results show that MCA691C is also suitable for use as a negative control with bovine, ovine, porcine, canine and guinea-pig tissues.

Target Species
Negative Control
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
*It is recommended that the user titrates the antibody for use in their system to a concentration equivalent to that of their test reagents.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

References for Mouse IgG2b Negative Control antibody

  1. Grant, A.J. et al. (2002) Hepatic expression of secondary lymphoid chemokine (CCL21) promotes the development of portal-associated lymphoid tissue in chronic inflammatory liver disease.
    Am J Pathol. 160 (4): 1445-55.
  2. Zheng, X. et al. (2002) Interleukin-3, but not granulocyte-macrophage colony-stimulating factor and interleukin-5, inhibits apoptosis of human basophils through phosphatidylinositol 3-kinase: requirement of NF-kappaB-dependent and -independent pathways.
    Immunology. 107 (3): 306-15.
  3. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  4. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382-94.
  5. Shoham, T. et al. (2001) Reduced expression of activin A in focal lymphoid agglomerates within nasal polyps.
    J Histochem Cytochem. 49 (10): 1245-52.

RRID
AB_322274
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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