CD14 antibody | MIL2
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Mouse anti Pig CD14:FITC
- Product Type
- Monoclonal Antibody
|Mouse anti Pig CD14, clone MIL2 recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001) . Mouse anti Pig CD14, clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition assays. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thacker et al. 2001) .
Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickova et al. 2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersen et al. 2007, Sanz et al. 2007).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Porcine peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml.
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the P2-X63-Ag.653 mouse myeloma.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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|Mouse IgG2b Negative Control:FITC||MCA691F||F||100 Tests||Log in|
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|Description||Mouse IgG2b Negative Control:FITC|
Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45.
References for CD14 antibody
Hauet, T. et al. (2000) Trimetazidine reduces renal dysfunction by limiting the cold ischemia/reperfusion injury in autotransplanted pig kidneys.
J Am Soc Nephrol. 11: 138-48.
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109.
Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
FASEB J. 24: 712-22.
Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
Kidney Int. 58: 838-50.
Li, Y. et al. (2014) Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus.
Virus Res. 189: 29-33.
Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology. 110: 440-9.
Vanderheijden, N. et al. (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages.
J Virol. 77: 8207-15.
Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
J Immunol. 187: 4913-9.
View The Latest Product References
Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
Kidney Int. 62: 654-67.
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94.
Thorgersen, E.B. et al. (2009) Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
Infect Immun. 77: 725-32.
Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
J Immunol Methods. 327: 18-29.
Facci, M.R. et al. (2011) Stability of expression of reference genes in porcine peripheral blood mononuclear and dendritic cells.
Vet Immunol Immunopathol. 141: 11-5.
Koutná, I. et al. (2012) Flow Cytometry Analysis of Intracellular Protein
In: Flow Cytometry - Recent Perspectives, Schmid, I. (Ed.), ISBN: 978-953-51-
Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
Immunology. 129: 396-405.
Schierack, P. et al. (2009) Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows.
Vet Immunol Immunopathol.127: 26-37.
Lundeland, B. et al. (2011) Severe gunshot injuries in a porcine model: impact on central markers of innate immunity.
Acta Anaesthesiol Scand. 55: 28-34.
Thorgersen, E.B. et al. (2008) Cyanobacterial LPS antagonist (CyP)-a novel and efficient inhibitor of Escherichia coli LPS-induced cytokine response in the pig.
Mol Immunol. 45: 3553-7.
Schierack, P. et al. (2007) Bacillus cereus var. toyoi enhanced systemic immune response in piglets.
Vet Immunol Immunopathol. 118: 1-11.
Ondrackova, P. et al. (2012) Interaction of porcine neutrophils with different strains of enterotoxigenic Escherichia coli.
Vet Microbiol. 160: 108-16.
Ondrackova, P. et al. (2013) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry.
Res Vet Sci. 94 (2): 240-5.
Vicenova, M. et al. (2014) Evaluation of in vitro and in vivo anti-inflammatory activity of biologically active phospholipids with anti-neoplastic potential in porcine model.
BMC Complement Altern Med. 14: 339.
Alvarez, B. et al. (2015) Phenotypic and functional heterogeneity of CD169+ and CD163+ macrophages from porcine lymph nodes and spleen.
Dev Comp Immunol. 44: 44-9.
Moffat, L. et al. (2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).
Dev Comp Immunol. 47 (1): 123-8.
Kyrova K et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
BMC Vet Res. 10: 244.
Nguyen, D.N. et al. (2016) Oral antibiotics increase blood neutrophil maturation and reduce bacteremia and necrotizing enterocolitis in the immediate postnatal period of preterm pigs.
Innate Immun. 22 (1): 51-62.
Egge, K.H. et al. (2015) Organ inflammation in porcine Escherichia coli sepsis is markedly attenuated by combined inhibition of C5 and CD14.
Immunobiology. 220 (8): 999-1005.
Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.
PLoS One. 11 (3): e0151256.
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832.
Zemankova, N. et al. (2016) Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages.
BMC Vet Res. 12 (1): 251.
Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
J Immunol. Nov 11. pii: 1600672. [Epub ahead of print]
Kavanová L et al. (2017) Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells.
Vet Res. 48 (1): 28.
Bacou, E. et al. (2017) β2-adrenoreceptor stimulation dampens the LPS-induced M1 polarization in pig macrophages.
Dev Comp Immunol. 76: 169-76.
Yang, G. et al. (2017) Characterizing porcine invariant natural killer T cells: A comparative study with NK cells and T cells.
Dev Comp Immunol. 76: 343-351.
Uitterdijk, A. et al. (2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.
PLoS One. 12 (6): e0178779.
Sánchez, E.G. et al. (2017) Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production.
Sci Rep. 7 (1): 10369.
Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
Dev Comp Immunol. 81: 95-104.
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92.
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91.
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
PLoS One. 14 (10): e0223647.
Vreman, S. et al. (2018) Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.
Dev Comp Immunol. 84: 361-70.
Lau, C. et al. (2020) NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies.
MAbs. 12 (1): 1686319.
Nielsen, O.L. et al. (2021) A porcine model of subcutaneous Staphylococcus aureus. infection: a pilot study.
APMIS. Mar 01 [Epub ahead of print].
Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
Vaccines. 10 (5): 684.
Štěpánová, H. et al. (2022) Characterization of Porcine Monocyte-Derived Macrophages Cultured in Serum-Reduced Medium.
Biology (Basel). 11(10):1457.
Monguió-Tortajada, M. et al. (2022) Acellular cardiac scaffolds enriched with MSC-derived extracellular vesicles limit ventricular remodelling and exert local and systemic immunomodulation in a myocardial infarction porcine model.
Theranostics. 12 (10): 4656-70.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
Petersen, C.B. et al. (2007) Cloning, characterization and mapping of porcine CD14 reveals a high conservation of mammalian CD14 structure, expression and locus organization.
Dev Comp Immunol. 31: 729-37.
Sanz, G. et al. (2007) Molecular cloning, chromosomal location, and expression analysis of porcine CD14.
Dev Comp Immunol. 31(7):738-47.
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