CD4 Alpha Antibody | MIL17

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CD4 Alpha Antibody | MIL17 gallery image 1

Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC (MCA1749F)

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CD4 Alpha Antibody | MIL17 gallery image 2

Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a (MCA1749GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD4 Alpha Antibody | MIL17 gallery image 3

Published customer image:
Mouse anti Porcine CD4 alpha, clone MIL17 used for the evaluation of CD4a expression onporcine peripheral blood mononuclear cells by flow cytometry.
Image caption:
T cell sub-population proliferation monitored by CFSE labelling in response to in vitro re-stimulation with H1N1 influenza. CFSE-labelled PBMC were cultured in vitro in the presence of 105EID50/ml inactivated Eng195, uninfected allantoic fluid (mock antigen), PWM or in media only. After 5 days, cells were stained with anti-CD4 and anti-CD8 monoclonal antibodies and analysed by flow cytometry for CFSE intensity. Three T cell subsets were identified on the basis of their CD4 and CD8 expression, and an example of the CFSE pattern at day 7pv in response to inactivated Eng195 in these various subsets in a pig vaccinated with the adjuvanted split vaccine is shown (A). At day 28pv, the percentage of cells proliferating (CFSELow cells) in response to media alone, mock antigen or inactivated Eng195 was determined after gating on CD4+CD8- (B), CD4+CD8+ (C) or CD4-CD8+ (D) cells. Results are expressed as the mean percentage of proliferating (CFSELow) cells ± SEM. n.s?=?Not statistically significant.

From: Lefevre EA, Carr BV, Inman CF, Prentice H, Brown IH, Brookes SM, et al. (2012)
Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes.
PLoS ONE 7(3): e32400.

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CD4 Alpha Antibody | MIL17 gallery image 4

Published customer image:
Mouse anti Porcine CD4 alpha, clone MIL17 used for the identification of CD4 expressing cells in procine jejunal mucosa by immunofluorescence.
Image caption:
Colonisation expands mucosal CD4+ T cells by 21 days of age. (A) CD4 staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to CD4 (green) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the blue channel (endothelium); (iii) shows the original greyscale image for the green channel (CD4). Scale bar represents 10 μm. (C) Area of CD4+ T cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). *p<0.05.

From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012)
Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.

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CD4 Alpha Antibody | MIL17 gallery image 5

Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD4a (MCA1749PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells

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CD4 Alpha Antibody | MIL17 gallery image 6

Figure A. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated Mouse anti Porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD4 Alpha Antibody | MIL17 gallery image 7

Figure A. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and Purified mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and purified Mouse anti Porcine CD25 (MCA1736GA) detected with Goat anti Mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD4 Alpha Antibody | MIL17 gallery image 8

Figure A. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD4 Alpha:FITC
  • Mouse anti Pig CD4 Alpha
  • Mouse anti Pig CD4 Alpha:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MIL17
  • Isotype
    IgG2b
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1749FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1749F
    MCA1749GAC *, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA1749GA
    MCA1749PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1749PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Porcine CD4 alpha, clone MIL17 recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.

      Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987)

      Mouse anti Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Leucocytes isolated from porcine gut lamina propria.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Alpha Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Alpha Antibody : Purified MIL17 C *, F 0.1 mg
    CD4 Alpha Antibody : RPE MIL17 F 100 Tests
    CD4 Alpha Antibody : FITC MIL17 F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      HCA038F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
      HCA038PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Pig CD45MCA1222GA0.1 mgC, F, IF
          MCA1222GA
          Mouse anti Pig wCD8 AlphaMCA1223GA0.1 mgC, F
          MCA1223GA
          Rat anti Human CD3MCA14770.2 mgC, F *, IF, P *, WB
          MCA1477

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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