Snapshot of eight T helper subsets
(PDF version available)
CD4 is perhaps the most crucial target of biomedical research today. Its role spans ground breaking T cell immunotherapy for cancer to HIV to several autoimmune diseases.
Also known as T4, and Leu-3, CD4 is a 55 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily. Primarily described as a marker for T cell subsets it can also be found on NKT cells, innate lymphoid cells and macrophages. It acts as a facilitator of the interaction between T cell receptor and the MHC/antigen complex on antigen presenting cells. It is this interaction that leads to T cell activation and also forms the basis of some T cell therapies against cancer.
CD4 forms part of the T cell receptor complex that binds to the beta 2 domain of the MHC molecule leading to activation of pathways downstream of the T cell receptor. Anti-CD4 monoclonal antibodies can also induce CD4 internalization which reduces overall CD4/MHC class II interactions, resulting in reduced T cell activation. CD4 is also a receptor for IL-16 (IL-16 stimulates migration response in CD4+ T cells and monocytes and induces CD4+ T cells to respond to IL-2 and IL-15) and a co-receptor for HIV and human herpes 7.
To learn more about CD4+ T cell subsets, take a look at our subtypes guide which provides a quick snapshot of the function of each T helper cell, as well as their biological profile. You can also access antibodies for studying these cells. The guide is intended to help you quickly identify the key information needed to design experiments to study these critical immune cells.
Our CD4 antibody range can be viewed here. Below we look at anti-CD4 clones and their recommended applications.
Anti-human CD4 V CD3
Red cell lysed human peripheral blood stained with CD4A647 (MCA1267A647) and CD3A488 (MCA463A488) in the presence of human seroblock (BUF070A)
Anti-mouse CD4 V CD3
Red cell lysed mouse spleen stained with CD4FITC (MCA2691F) and CD3PE (MCA500PE) in the presence of mouse seroblock (BUF041A)
Immunoperoxidase staining of a human tonsil cryosection with mouse anti-human CD4 antibody, clone RPA-T4 (MCA1267) followed by visualization using the Histar Detection System (STAR3000)
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Searching for your antibody of choice is made easy by the use of the sophisticated filter table, which allows you to select for specific antibody parameters. If you are unable to find your desired antibody simply contact us as we are adding new product all the time and we will be happy to help advise you.