Mouse IgG2b Negative Control Antibody

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Mouse IgG2b Negative Control Antibody gallery image 1

Figure A. FITC conjugated Mouse anti Human CD19 (MCA2495F) and A647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. FITC conjugated Mouse anti Human CD19 (MCA2495F) and A647 conjugated Mouse anti Human CD20 (MCA1710A647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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Mouse IgG2b Negative Control Antibody gallery image 2

Figure A. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse anti Human CD9 (MCA469PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A)

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Mouse IgG2b Negative Control Antibody gallery image 3

Figure A. RPE-A750 tandem conjugated Mouse anti Human CD3 (MCA463P750) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. RPE-A750 tandem conjugated Mouse anti Human CD3 (MCA463P750) and RPE conjugated Mouse anti Human CD52 (MCA2188PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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Mouse IgG2b Negative Control Antibody gallery image 4

Figure A. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated Mouse anti Porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 5

Figure A. Purified Mouse anti Porcine CD25 (MCA1736GA) detected with Goat anti Mouse IgG1 PE (STAR132PE) and Mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified Mouse anti Porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and Mouse anti Porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 6

Figure A. Purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD11a FITC (MCA2308F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 7

Figure A. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse anti porcine CD18a (MCA1972F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 8

Figure A. Purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a PE (STAR133PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD6 (MCA1221GA) detected with goat anti mouse IgG2a PE (STAR133PE) and mouse anti porcine CD45 FITC (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 9

Figure A. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD14 (MCA1218F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 10

Figure A. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified mouse IgG2b isotype control (MCA691) detected with RPE conjugated goat anti mouse IgG2b (STAR134PE). Figure B. FITC conjugated mouse anti bovine CD40 (MCA2431F) and purified CD1w3 (MCA1647G) detected with RPE conjugated goat anti mouse IgG2b (MCASTAR134PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 11

Figure A. RPE conjugated mouse anti bovine CD8b (MCA1654PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti bovine CD8b (MCA1654PE) and FITC conjugated mouse anti bovine CD21 (MCA5953F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Mouse IgG2b Negative Control Antibody gallery image 12

Figure A. Purified mouse anti bovine CD13 (MCA2338GA) detected with goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. Purified mouse anti bovine CD13 (MCA2338GA) detected with goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated mouse anti bovine CD11b (MCA1425F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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  • Mouse IgG2b Negative Control:RPE-Alexa Fluor® 647
  • Mouse IgG2b Negative Control:RPE-Cy5
  • Mouse IgG2b Negative Control:APC
  • Mouse IgG2b Negative Control:Pacific Blue®
  • Mouse IgG2b Negative Control:RPE
  • Mouse IgG2b Negative Control:Biotin
  • Mouse IgG2b Negative Control:Alexa Fluor® 700
  • Mouse IgG2b Negative Control:Alexa Fluor® 488
  • Mouse IgG2b Negative Control:Alexa Fluor® 647
  • Mouse IgG2b Negative Control
  • Mouse IgG2b Negative Control:Preservative Free
  • Mouse IgG2b Negative Control:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
    Mouse IgG2b isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a rat surface antigen.
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgG2b
    12 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA691XZC, F, P, R, REdatasheet pdfdatasheet pdf1 mg
      MCA691XZ
      MCA691CFdatasheet pdfdatasheet pdf100 Tests
      MCA691C
      MCA691APCFdatasheet pdfdatasheet pdf100 Tests
      MCA691APC
      MCA691PEFdatasheet pdfdatasheet pdf100 Tests
      MCA691PE
      MCA691BFdatasheet pdfdatasheet pdf100 Tests
      MCA691B
      MCA691Fdatasheet pdfdatasheet pdf100 Tests
      MCA691
      MCA691FFdatasheet pdfdatasheet pdf100 Tests
      MCA691F
      MCA691P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA691P647
      MCA691PBFdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA691PB
      MCA691A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA691A700
      MCA691A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA691A488
      MCA691A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA691A647
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse IgG2b Negative Control is negative on all human cells and cell lines tested. This antibody recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

        Test results show that MCA691C is also suitable for use as a negative control with bovine, ovine, porcine, canine and guinea-pig tissues.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
        Purified IgG conjugated to R Phycoerythrin (RPE) Cy5 - liquid
        Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
        Purified IgG conjugated to Pacific Blue® - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      • Reconstitution
        Reconstitute with 1 ml distilled water
        Reconstitute with 1.0ml distilled water
        Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
        Reconstitute with 1 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.2%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline.
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        This product should be stored undiluted.

        DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        This product should be stored undiluted.

        DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC for one month or at -20oC for longer.

        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of reconstitution.
        6 months from date of despatch.
        6 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
        Cy® and CyDye® are registered trademarks of GE Healthcare
        The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
        Immunohistology - Frozen*
        Immunohistology - Paraffin*
        Immunohistology - Resin*
        Radioimmunoassays*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
        *It is recommended that the user titrates the antibody for use in their system to a concentration equivalent to that of their test reagents.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                References

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