T Lymphocytes antibody | KEN-5




In contrast to humans and mice where only a very small population of B-cells express the CD5 antigen, the CD5 antigen in rabbits is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognising rabbit CD5 (Kotani et al.1993), does not bind to rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognizing rabbit CD5 and anti human cross reactive CD5 antibodies, shows very limited binding to rabbit B-cells, which in adult rabbits have been demonstrated to express CD5 (Raman & Knight 1992) More recently studies (Pospisil et al. 2009) indicate that clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. It has therefore been suggested that KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells (Pospisil et al. 2009).
Product Details
- Target Species
- Rabbit
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rabbit Thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the PAI mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of T Lymphocytes antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
ELISA | |||
Flow Cytometry | 1/50 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat anti Human CD3 | MCA1477 | C F* IF P* WB | 0.2 mg |
![]() |
|
Mouse anti Human CD14 | MCA1568GA | E F IF | 0.1 mg |
![]() |
|
Mouse anti Rabbit CD8 | MCA1576GA | F | 0.1 mg |
![]() |
|
Mouse anti Human CD79a | MCA2538GA | C F* P* | 0.1 mg |
![]() |
|
Mouse anti Rabbit CD4 | MCA799GA | C F IP | 0.1 mg |
![]() |
|
Mouse anti Rabbit CD45 | MCA808GA | C F IF | 0.1 mg |
![]() |
Product Specific References
References for T Lymphocytes antibody
-
Kotani, M. et al. (1993) Generation and characterization of monoclonal antibodies against rabbit CD4, CD5 and CD11a antigens.
J Immunol Methods. 157 (1-2): 241-52. -
Raman, C. & Knight, K.L. (1992) CD5+ B cells predominate in peripheral tissues of rabbit.
J Immunol. 149 (12): 3858-64. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLoS One. 3 (2): e1627. -
Guerrero, I. et al. (2010) Evolution of the peripheral blood lymphocyte populations in multiparous rabbit does with two reproductive management rhythms.
Vet Immunol Immunopathol. 140: 75-81. -
Gillet, L. et al. (2009) Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity.
Vet Res. 40: 51. -
Matsumura, T. et al. (1999) Suppression of atherosclerotic development in Watanabe heritable hyperlipidemic rabbits treated with an oral antiallergic drug, tranilast.
Circulation. 99 (7): 919-24. -
Hoefer, I.E. et al. (2005) Leukocyte subpopulations and arteriogenesis: specific role of monocytes, lymphocytes and granulocytes.
Atherosclerosis. 181 (2): 285-93. -
Gu, W. et al. (2004) Immune response in rabbit ovaries following infection of a recombinant myxoma virus expressing rabbit zona pellucida protein B.
Virology. 318 (2): 516-23. -
Milanovic, V. et al. (2017) Histological and immunological changes in uterus during the different reproductive stages at Californian rabbit (Oryctolagus cuniculus).
Kafkas Univ Vet Fak Derg, 23, 137-44. -
Gates, K.V. & Griffiths, L.G. (2018) Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial.
Immunol Res. 66 (2): 288-98. -
Penadés, M. et al. (2018) Long-term implications of feed energy source in different genetic types of reproductive rabbit females. II. Immunologic status.
Animal. 12 (9): 1877-85. -
Penadés, M.et al. (2019) Early deviations in performance, metabolic and immunological indicators affect stayability in rabbit females.
Animal. : 1-10. -
Niedźwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
Pol J Vet Sci. 23 (1): 37-42.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra