CD45RC antibody | 3a56

Mouse anti Pig CD45RC

Product Type
Monoclonal Antibody
Clone
3a56
Isotype
IgG1
Specificity
CD45RC

Product Code Applications Pack Size List Price Your Price Qty
MCA6049GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Pig CD45RC, clone 3a56 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will detect the ~210 kDa RC isoform.

CD45 in pigs has three cell surface expressed isoforms: CD45R, CD45RA, and CD45RC (Schnitzlein and Zuckermann 1998). CD45RC is mainly expressed by CD4+ T cells and weakly by B cells (Piriou-Guzylack and Salmon 2008). However, during the immune response expression of CD45RC was found to decrease in activated T-helper cells (Saalmuller et al. 2002). In contrast, increased expression of CD45RC was observed during the maturation of CD4- gamma delta thymocytes (Sinkora et al. 2005).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Further Reading

  1. Zuckermann, F.A. et al. (2001) Characterization of monoclonal antibodies assigned to the CD45 subgroup of the Third International Swine CD Workshop.
    Vet Immunol Immunopathol. 80 (1-2): 165-74.
  2. Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39 (6): 54.
  3. Schnitzlein, W.M. & Zuckermann, F.A. (1998) Determination of the specificity of CD45 and CD45R monoclonal antibodies through the use of transfected hamster cells producing individual porcine CD45 isoforms.
    Vet Immunol Immunopathol. 60 (3-4): 389-401.
  4. Saalmüller, A. et al. (2002) T-helper cells from naive to committed.
    Vet Immunol Immunopathol. 87 (3-4): 137-45.
  5. Sinkora, M. et al. (2005) Development of gammadelta thymocyte subsets during prenatal and postnatal ontogeny.
    Immunology. 115 (4): 544-55.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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